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Thromb Haemost 1979; 42(04): 1230-1239
DOI: 10.1055/s-0038-1657018
Original Article
Schattauer GmbH Stuttgart

In Vivo Recovery of Factor VIII: A Comparison of One-Stage and Two-Stage Assay Methods

I M Nilsson
The Coagulation Laboratory, General Hospital, Malmö, Sweden
,
T B L Kirkwood
*   National Institute for Biological Standards and Control, London NW3 6RB, England
The Coagulation Laboratory, General Hospital, Malmö, Sweden
,
T W Barrowcliffe
*   National Institute for Biological Standards and Control, London NW3 6RB, England
The Coagulation Laboratory, General Hospital, Malmö, Sweden
› Author Affiliations
Further Information

Publication History

Received 12 December 1978

Accepted 10 February 1979

Publication Date:
23 August 2018 (online)

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Summary

The recovery and half-life of VIII: C in the plasma of severely haemophilic patients was measured by one-stage and two-stage assays after injection of two Factor VIII concentrates (Hemofil, Hyland and Fraction I-O, Kabi). Plasma volumes were measured with an Evans� Blue technique, and both concentrates and post-infusion samples were measured against the same plasma standard.

There was a highly significant difference in recoveries estimated by the two assay methods. The one-stage assays gave the most consistent results, in that the average recovery was 100%, whereas the two-stage assays gave only about 80% of the value expected from in vitro assays. There was no difference in recoveries between the two concentrates.

The two-stage assays gave a slightly shorter half-life than the one-stage assays, and the half-life of Hemofil was also shorter than that of Fraction I-O.

 

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