Relative Activities of Heparin Fractions Obtained by Gel Chromatography

Summary

In order to investigate the reported heterogeneity of commercial heparin injections, lung and mucous preparations were fractionated by gel chromatography according to molecular size. Eluent pools were characterized by measuring electrophoretic mobility, anticoagulant activity using a reactivation assay and an anti-Xa assay, lipolytic activity and the protamine neutralization value. The only biological activity to show a marked relationship with the molecular size of the heparin fraction was the anti-Xa activation activity. This effect was more pronounced with the mucous heparins than with the lung heparin preparation studied.

• References

• 1 Barrowcliffe TW, Johnson EA, Eggleton CA, Thomas DP. 1977; Anticoagulant activities of lung and mucous heparins. Thrombosis Research 12: 27
• 2 Bianchini P, Guidi G, Osima B. 1972; Blood levels of the clearing factor after administration of a natural glucuronoglucosaminoglycan (3GS). Biochemistry and Experimental Biology. 10: 243
• 3 Denson KW E, Bonnar J. 1973; The measurement of heparin. A method based on the potentiation of anti-factor Xa Thrombosis et Diathesis Haemorrhagica 30: 471
• 4 Hall M, Ricketts CR, Michael SE. 1969; The potency of heparin fractions obtained by gel filtration. Journal of Pharmacy and Pharmacology 21: 626
• 5 Johnson EA, Mulloy B. 1976; The molecular-weight range of mucosal-heparin preparations. Carbohydrate Research 51: 1-19
• 6 Kavanagh LW, Jaques LB. 1973; Comparison of analytical values for commercial heparins. Arzneimittel-Forschung (Drug Research) 23: 605
• 7 Lane DA, MacGregor IR, Michalski R, Kakkar VV. 1978; Anticoagulant activities of four unfractionated and fractionated heparins. Thrombosis Research 12: 257
• 8 Lasker SE, Stivala SS. 1966; Physicochemical studies of fractionated bovine heparin. Archives of Biochemistry and Biophysics 115: 360
• 9 Laurent TC. 1961; Studies on fractionated heparin. Archives of Biochemistry and Biophysics 92: 224
• 10 Mcduffie NM, Dietrich CP, Nadar HB. 1975; Electrofocusing of heparin: fractionation of heparin into 21 components distinguishable from other acidic mucopolysaccharides. Biopolymers 14: 1473
• 11 Sugisaka N, Petracek FJ. 1977; Rapid molecular size determination of heparins by high pressure liquid chromatography. Federation Proceedings 26: 89
• 12 Walton PL, Wright BM. 1964; An automatic coagulometer. Journal of Physiology (London) 172: 7 P