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Thromb Haemost 1979; 42(05): 1615-1619
DOI: 10.1055/s-0038-1657065
Original Article
Schattauer GmbH Stuttgart

Human Platelet Acetylcholinesterase: The Effects of Anticholinesterases on Platelet Function

Martin J Smith
The Gundersen Clinic, Ltd. and La Crosse Lutheran Hospital, La Crosse, Wisconsin, U.S.A.
,
Boyd Braem
The Gundersen Clinic, Ltd. and La Crosse Lutheran Hospital, La Crosse, Wisconsin, U.S.A.
,
Kent D Davis
The Gundersen Clinic, Ltd. and La Crosse Lutheran Hospital, La Crosse, Wisconsin, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 02 May 1979

Accepted 11 December 1979

Publication Date:
18 February 2019 (online)

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Summary

Platelet acetylcholinesterase (AChE) activity was measured in gel-filtered platelet preparations. Three different anticholinesteratic agents (eserine, neostigmine, and diiso- propylphosphorofluoridate) at final concentrations of 10 μM caused complete inhibition of AChE activity after 30 min incubation at room temperature with either platelet-rich plasma or gel-filtered platelets. Complete inhibition of platelet AChE had no effect on platelet aggregation, factor-3 availability, and plasma clot retraction. We conclude that platelet membrane AChE activity is not required for normal platelet function as measured by these in vitro parameters.

 

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