Thromb Haemost 1982; 47(01): 072-075
DOI: 10.1055/s-0038-1657130
Original Article
Schattauer GmbH Stuttgart

A Comparison of the Bethesda and New Oxford Methods of Factor VIII Antibody Assay

D E G Austen
1   The Haemophilia Centre, Churchill Hospital, Oxford, England
,
K Lechner
2   The Medizinische Universitätsklinik, Vienna, Austria
,
C R Rizza
1   The Haemophilia Centre, Churchill Hospital, Oxford, England
,
I L Rhymes
1   The Haemophilia Centre, Churchill Hospital, Oxford, England
› Author Affiliations
Further Information

Publication History

Received 21 September 1981

Accepted 14 January 1982

Publication Date:
13 July 2018 (online)

Summary

A collaborative trial has been carried out under the auspices of the International Committee on Thrombosis and Haemostasis to compare the Bethesda and New Oxford methods of antibody assay. It was found that errors between laboratories were much greater than those within laboratories and each laboratory had a bias whereby it always rated samples high or low with respect to the other laboratories. However there was excellent agreement in the order in which laboratories ranked antibody samples and if a standard antibody sample could be provided there would be a significant improvement in numerical agreement between laboratories. On average, for this exercise, a result for a given sample in Bethesda units was 1.21 times the result in New Oxford units although it must be stressed that this ratio could vary from sample to sample.

 
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