Partial Characterization of a Fibrinolytic Inhibitor Produced by Cultured Endothelial Cells Derived from Human Umbilical Vein

F Esnard; E Dupuy; A M Dosne; E Bodevin

doi:10.1055/s-0038-1657146

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1982; 47(02): 128-131
DOI: 10.1055/s-0038-1657146

Original Article

Schattauer GmbH Stuttgart

Partial Characterization of a Fibrinolytic Inhibitor Produced by Cultured Endothelial Cells Derived from Human Umbilical Vein

Authors

F Esnard

The Laboratory of Biochemistry, Faculty of Medicine, Tours, France, and from INSERM Unit 150, HÃ´pital LariboisiÃ¨re, Paris, France
E Dupuy

The Laboratory of Biochemistry, Faculty of Medicine, Tours, France, and from INSERM Unit 150, HÃ´pital LariboisiÃ¨re, Paris, France
A M Dosne

The Laboratory of Biochemistry, Faculty of Medicine, Tours, France, and from INSERM Unit 150, HÃ´pital LariboisiÃ¨re, Paris, France
E Bodevin

The Laboratory of Biochemistry, Faculty of Medicine, Tours, France, and from INSERM Unit 150, HÃ´pital LariboisiÃ¨re, Paris, France

Abstract

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Summary

A preliminary characterization of a fibrinolytic inhibitor released by human umbilical vein endothelial cells in primary culture is reported. This molecule of Mr comprised between 2 × 10⁵ and 10⁶ and of μ₂ mobility precipitates at 43% ammonium sulphate saturation and is totally adsorbed on Concanavalin A Sepharose 4 B. A possible relationship with a macroglobulins is discussed.

Key words

Fibrinolysis inhibition - Urokinase - Endothelial cells - Umbilical vein

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References

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