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Thromb Haemost 1982; 47(03): 193-196
DOI: 10.1055/s-0038-1657166
Original Article
Schattauer GmbH Stuttgart

The Amidolytic Activity of the SK-Plasminogen Complex Is Enhanced by a Potentiator which Is Generated in the Presence of Vascular Plasminogen Activator - Role of Fibrin Degradation Products

J Soria
1   The Laboratoires Centraux de Biochimie et d'Hématologie, (Pr. Fabiani, Pr. Samama), Service du Pr. Bernadou, Hôtel Dieu, Parvis de Notre Dame, Paris, France
,
C Soria
2   The Laboratoires Centraux de Biochimie et d'Hématologie, (Pr. Rousselet, Pr. Caen), Hôpital Lariboisière, Paris
,
O Bertrand
3   The Inserm U 160 (Service du Prof. Boivin), Hôpital Beaujon Clichy
,
F Dunn
1   The Laboratoires Centraux de Biochimie et d'Hématologie, (Pr. Fabiani, Pr. Samama), Service du Pr. Bernadou, Hôtel Dieu, Parvis de Notre Dame, Paris, France
2   The Laboratoires Centraux de Biochimie et d'Hématologie, (Pr. Rousselet, Pr. Caen), Hôpital Lariboisière, Paris
,
M Samama
1   The Laboratoires Centraux de Biochimie et d'Hématologie, (Pr. Fabiani, Pr. Samama), Service du Pr. Bernadou, Hôtel Dieu, Parvis de Notre Dame, Paris, France
,
F Bachmann
4   The C.H.U.V., Laboratoire Central d'Hématologie, Lausanne, Switzerland
› Author Affiliations
Further Information

Publication History

Received 05 October 1981

Accepted 15 February 1982

Publication Date:
13 July 2018 (online)

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Summary

In the presence of an excess of streptokinase (SK) the amidolytic activity of the plasminogen-SK complex on chromogenic substrates is 12% lower in serum than in the corresponding plasma. However, in subjects in whom venous stasis lead to a shortening of the euglobulin lysis time to less than 60 min (high responders), the amidolytic activity of the plasminogen-SK complex in serum was 60% higher than in the corresponding plasma. Attempts to find alterations of the plasminogen molecule itself which would account for the enhanced activity in high responder serum were negative. No free plasmin was present and the plasminogens isolated from plasma and serum before and after venous stasis had the same amidolytic activity as glu-plasminogen in the presence of an excess of SK. N-terminal analysis of these four plasminogens revealed in each instance glutamic acid.

The enhancement of the amidolytic activity of the SK-plasminogen complex in serum of high responders (potentiator activity) could be reproduced by adding purified tissue plasminogen activator (TA) to native blood before clotting, but not if TA was added to plasma or to prestasis serum. Removal of fibrin degradation products from poststasis serum resulted in the disappearance of potentiator activity. These experiments suggest that fibrin degradation products, generated during clotting in the presence of vascular or tissular plasminogen activator act as a potentiator of the amidolytic activity of the plasminogen SK-complex.

Key words

Plasminogen - SK-plasminogen complex - Fibrinolysis - Potentiator of SK-plasminogen complex - Tissue plasminogen activator - Venous stasis test - Fibrin degradation products
 

  • References

  • 1 Robbins KC, Markus G. The interaction of human plasminogen with streptokinase. In Fibrinolysis Current Fundamental and Clinical Concepts. Gaffney PJ, Balkuv-Ulutin S. (Ed.) Academic Press; New York: 1978: 61-75
    Search in Google Scholar
  • 2 Soria J, Soria C, Samama M. A plasminogen assay using a chromogenic synthetic substrate: results from clinical work and from studies of thrombolysis. In: Progress in Chemical Fibrinolysis and Thrombolysis. Vol. 3 Davidson JF, Samama M, Desnoyers PC. (Ed.) Raven Press; New York: 1978: 337-346
    Search in Google Scholar
  • 3 Rijken DC, Wijngaard SG, Welbergen J. Relationship between tissue plasminogen activator and the activators in blood and vascular wall. Thromb Res 1980; 18: 815-830
    CrossrefPubMedSearch in Google Scholar
  • 4 Nilsson IM, Pandolfi M. Assay of fibrinolytic activity of the vessel wall. In: Progress in Chemical Fibrinolysis and Thrombolysis. Vol. 2 Davidson JF, Samama M, Desnoyers PC. (Ed.) Raven Press; New York: 1976: 1-13
    Search in Google Scholar
  • 5 Soria C, Rodriguez-Zeballos A, Soria J, Kartalis G. Plasminogen activity in plasma and in serum before and after venostasis. Thromb Haemostas 1979; 42: 288
    PubMedSearch in Google Scholar
  • 6 Soria C, Soria J, Ryckewaert JJ, Rodriguez-Zeballos A, Samama M. Influence of coagulation and endothelial plasminogen activator on plasminogen biological activity. VI Congrès International sur la Thrombose de la Ligue Méditerranéenne, Monte Carlo. 1980. (Abstr. 152)
    Search in Google Scholar
  • 7 Hjelm H, Hjelm K, Sjoquist J. Protein A from staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins. Febs Lett 1972; 28: 73-76
    CrossrefPubMedSearch in Google Scholar
  • 8 Deutsch DG, Mertz ET. Plasminogen: purification from human plasma by affinity chromatography. Science 1970; 170: 1095-1096
    CrossrefPubMedSearch in Google Scholar
  • 9 Tan-Wilson AL, Reichlin M, Noble RW. Isolation and characterization of low and high affinity goat antibodies directed to simple antigenic sites on human hemoglobin. Immunochemistry 1976; 13: 921-927
    CrossrefPubMedSearch in Google Scholar
  • 10 Friberger P, Knos M. Plasminogen determination in human plasma. In: Chromogenic Peptide Substrates. Chemistry and Clinical Usage. Scully MF, Kakkar VV. (Ed.) Churchill Livingstone; London: 1979: 128-140
    Search in Google Scholar
  • 11 Mancini G, Carbonara AO, Hermans JF. Immunological quantitation of antigens by single radial immunodiffusion. Immunochemistry 1965; 2: 235-254
    CrossrefPubMedSearch in Google Scholar
  • 12 Weber K, Osborn M. The reliability of molecular weight determination by SDS-polyacryliamide gel electrophoresis. J Biol Chem 1969; 244: 4406-4412
    PubMedSearch in Google Scholar
  • 13 Weiner AM, Platt T, Weber K. Aminoterminal sequence analysis of proteins purified on a nanomole scale by gel electrophoresis. J Biol Chem 1972; 247: 3242-3251
    PubMedSearch in Google Scholar
  • 14 Woods KR, Wang KT. Separation of dansyl-aminoadds by polyamide layer chromatography. Biochim Biophys Acta 1967; 133: 366-369
    CrossrefPubMedSearch in Google Scholar
  • 15 Caen J, Larrieu MJ, Samama M. Lyse des euglobulines plasmatiques. In: L’hémostase. Méthodes d’exploration et diagnostic pratique. Expansion Scientifique Française; Paris: 1975: 256
    Search in Google Scholar
  • 16 Wallén P, Ranby M. Interaction of fibrin and tissue plasminogen activator. Thromb Haemostas 1981; 46: 252 ff. (Abstr.)
    PubMedSearch in Google Scholar
  • 17 Camiolo SM, Markus G, Evers JL, Hobika GH. Augmentation of streptokinase activator activity by fibrinogen of fibrin. Thromb Res 1980; 17: 697-706
    CrossrefPubMedSearch in Google Scholar
  • 18 Takada AK, Muchizuki K, Takada Y. Further characterization of SK-potentiators of plasminogen. Thromb Res 1980; 19: 485-492
    CrossrefPubMedSearch in Google Scholar