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Thromb Haemost 1982; 48(01): 009-012
DOI: 10.1055/s-0038-1657204
Original Article
Schattauer GmbH Stuttgart

6-oxo-Prostaglandin F1α and Thromboxane B2 in Uterine Vein Blood - A Possible Role in Menstrual Bleeding

C F Goodfellow
1   The University Dept. of Obstetrics, Leeds, U. K.
,
R C Paton
2   The University Dept. of Medicine, General Infirmary, Leeds, U. K.
,
J A Salmon
3   The Dept. of Prostaglandin Research, Wellcome Research Laboratories, Beckenham, U. K.
,
S Moncada
3   The Dept. of Prostaglandin Research, Wellcome Research Laboratories, Beckenham, U. K.
,
J K Clayton
4   The St. Luke's Hospital, Bradford, U. K.
,
J A Davies
2   The University Dept. of Medicine, General Infirmary, Leeds, U. K.
,
G P McNicol
University of Aberdeen, Aberdeen, U. K.
› Author Affiliations
Further Information

Publication History

Publication Date:
28 August 2018 (online)

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Summary

The role of the haemostatic system in relation to menstrual bleeding is poorly understood. Platelet retention to glass beads and plasma concentrations of 6-oxo-PGFα and thromboxane B2 were measured in uterine and peripheral venous blood obtained from 18 women undergoing abdominal hysterectomy. Concentrations of 6-oxo-PGFα were significantly (p<0.01) higher in uterine (1.4 ± 0.3 ng/ml, mean ± SEM) than in peripheral vein blood (0.2 ±0.1 ng/ml) as was the level of thromboxane B2 (0.5 + 0.1 and 0.2 ± 0.1 ng/ml, respectively). Platelet retention in uterine vein blood (11 ± 4%) was significantly lower than in peripheral blood (42 ±4%; p<0.01) and the degree of platelet retention correlated inversely with the plasma concentration of 6-oxo-PGFα (r −0.43; p<0.01). There was a significant rank correlation between time since menstruation and concentrations of 6-oxo-PGFα in uterine (τ + 0.69; p<0.001) and peripheral (τ + 0.56; p<0.05) vein blood. The results indicate that an increased local production of prostacyclin (PGI2) relative to thromboxane A2 at the time of menstruation could contribute to the mechanism of uterine bleeding.

Keywords

Prostacyclin - Thromboxane B2 - Uterus - Menstruation - Platelets
 

  • References

  • 1 Quick AJ. Menstruation in hereditary bleeding disorders. Obstet Gynecol 1966; 28: 37-38
    PubMedGoogle Scholar
  • 2 Anderson ABM, Haynes PJ, Guillebaud J, Turnbull AC. Reduction of menstrual blood loss by prostaglandin synthetase inhibitors. Lancet 1976; 1: 774-776
    PubMedGoogle Scholar
  • 3 Guillebaud J, Anderson AB, Turnbull AC. Reduction by mefanamic acid of increased menstrual blood-loss associated with intrauterine contraception. Br J Obstet Gynaecol 1978; 85: 53-62
    CrossrefPubMedGoogle Scholar
  • 4 Ylikorkala O, Kauppila A, Siljander M. Anti-prostaglandin therapy in prevention of side-effects of intrauterine contraceptive devices. Lancet 1979; 2: 393-394
    PubMedGoogle Scholar
  • 5 Hagenfeldt K. Prostaglandins and related compounds and their metabolism in normal and steroid-exposed endometrium. Diczfalusy E, Fraser IS, Webb FTG. (eds.). Endometrial bleeding and steroidal contraception. Bath, England: Pitman Press; 1980: 222-239
    Google Scholar
  • 6 Abel MH, Kelly RW. Differential production of prostaglandins within the human uterus. Prostaglandins 1979; 18: 821-828
    CrossrefPubMedGoogle Scholar
  • 7 Liggins GC, Campos GA, Roberts CM, Skinner SJ. Production rates of prostaglandin F, 6-keto-PGF1α and thromboxane B2 by perfused human endometrium. Prostaglandins 1980; 19: 461-467
    CrossrefPubMedGoogle Scholar
  • 8 Mustard JF, Kinlough-Rathbone RL, Packham MA. Prostaglandins and platelets. Annu Rev Med 1980; 31: 89-96
    CrossrefPubMedGoogle Scholar
  • 9 Ponari O, Civardi E, Megha S, Pini M, Portioli D, Dettori AG. Antiplatelet effects of long-term treatment with gliclazide in diabetic patients. Thromb Res 1979; 16: 191-203
    CrossrefPubMedGoogle Scholar
  • 10 Salmon JA. A radioimmunoassay for 6-keto-prostaglandin F1α . Prostaglandins 1978; 15: 383-397
    CrossrefPubMedGoogle Scholar
  • 11 Higgs GA, Salmon JA. Cyclooxygenase products in carrageenin-induced inflammation. Prostaglandins 1979; 17: 737-746
    CrossrefPubMedGoogle Scholar
  • 12 Nossel HL. Radioimmunoassay of fibrinopeptides in relation to intravascular coagulation and thrombosis. N Engl J Med 1976; 295: 428-432
    CrossrefPubMedGoogle Scholar
  • 13 Ingerman-Wojenski C, Silver MJ, Smith JB, Macarak E. Bovine endothelial cells in culture produce thromboxane as well as prostacyclin. J Clin Invest 1981; 67: 1292-1296
    CrossrefPubMedGoogle Scholar
  • 14 Gimeno MF, Berda ES, Lazzari MA, Gimeno AL. Ovarian hormones inhibit the release of prostacyclin-like material from isolated rat uterus. Prostaglandins 1980; 20: 223-232
    CrossrefPubMedGoogle Scholar
  • 15 Hohman WR, Shaw ST, Macaulay L, Moyer DL. Vascular defects in human endothelium caused by intrauterine contraceptive devices. Contraception 1977; 16: 507-522
    CrossrefPubMedGoogle Scholar
  • 16 Aparicio SR, Bradbury K, Bird CC, Foley ME, Jenkins DM, Clayton JK, Scott JS, Rajah SM, McNicol GP. Effect of intrauterine contraceptive devices on uterine haemostasis: a morphological study. Br J Obstet Gynaecol 1979; 86: 314-324
    CrossrefPubMedGoogle Scholar
  • 17 Christiaens GCML, Sixma JJ, Haspels AA. Morphology of haemostasis in menstrual endometrium. Br J Obstet Gynaecol 1980; 87: 425-439
    CrossrefPubMedGoogle Scholar
  • 18 Smith JB, Ingelman CM, Silver MJ. Malondialdehyde formation as an indicator of prostaglandin production by human platelets. J Lab Clin Med 1976; 88: 167-172
    PubMedGoogle Scholar
  • 19 Tindall H, Zuzel M, Paton RC, McNicol GP. Changes in thrombo-stimulated platelet malondialdehyde production during the menstrual cycle. J Clin Pathol 1981; 34: 595-598
    CrossrefPubMedGoogle Scholar
  • 20 Smith SK, Abel MH, Kelly RW, Baird DT. A role for prostacyclin (PGI2) in excessive menstrual bleeding. Lancet 1981; 1: 522-524
    PubMedGoogle Scholar