Thromb Haemost 1982; 48(01): 033-037
DOI: 10.1055/s-0038-1657210
Original Article
Schattauer GmbH Stuttgart

Reactivity of Fibrinogen Derivatives with Antisera to Human Fibrin D-Dimer and Its γ-γ Chain Remnant

C S Cierniewski
The Department of Biophysics, Institute of Physiology and Biochemistry, Medical School of Lodz, Lodz, Poland
,
A Janiak
The Department of Biophysics, Institute of Physiology and Biochemistry, Medical School of Lodz, Lodz, Poland
,
P Nowak
The Department of Biophysics, Institute of Physiology and Biochemistry, Medical School of Lodz, Lodz, Poland
,
W Augustyniak
The Department of Biophysics, Institute of Physiology and Biochemistry, Medical School of Lodz, Lodz, Poland
› Author Affiliations
Further Information

Publication History

Received 13 July 1981

Accepted 11 May 1982

Publication Date:
13 July 2018 (online)

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Summary

Highly purified D-dimer was obtained from plasmin digest of human cross-linked fibrin. After reduction of its disulfide bonds, the γ-γ chain remnant, containing cross-linking site, was then isolated by ion-exchange chromatography on CM-cellulose. Antisera obtained by immunizing rabbits with D-dimer and its γ-γ chain remnant contained a small population of antibodies which specifically reacted with D-dimer. Thus, a specific radioimmunoassay system allowing detection and quantitation of D-dimer in the presence of fibrinogen and monomeric fragment D was made possible.