Download PDF
Thromb Haemost 1982; 48(01): 087-090
DOI: 10.1055/s-0038-1657223
Original Article
Schattauer GmbH Stuttgart

Assessment of Antithrombotic Properties of Sodium Ibuprofen

Carlos O Esquivel
*   The Dept. of Surgery, University of California Davis, California, U.S.A.
,
David Bergqvist
**   The Dept. of Experimental Research, Malmö General Hospital, University of Lund, Malmö, Sweden
,
Claes-Göran Björck
**   The Dept. of Experimental Research, Malmö General Hospital, University of Lund, Malmö, Sweden
,
Stan N Carson
*   The Dept. of Surgery, University of California Davis, California, U.S.A.
,
Bodil Nilsson
**   The Dept. of Experimental Research, Malmö General Hospital, University of Lund, Malmö, Sweden
› Author Affiliations
Further Information

Publication History

Received 22 April 1982

Accepted 16 June 1982

Publication Date:
13 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

The effect of sodium ibuprofen on platelet activity in vivo and the lysability of ex vivo thrombi was investigated. The formation of a hemostatic platelet plug in the rabbit mesentery and platelet embolism as a response to a laser-induced injury in the ear chamber of rabbits were used as models for determining platelet activity. Ibuprofen at a dose of 25 mg/kg i.v. was found to increase the primary (PHT) and the total hemostatic plug formation time (THT). The same dose decreased the number of cumulative emboli over a 10 min period after a laser injury to arterioles. A dose of 10 mg/kg i.v. did not affect the formation of the hemostatic platelet plug. In dogs, doses of 10, 25 und 50 mg/kg did not enhance the release of 125I-FDP from the thrombi after incubation in plasmin, but the largest dose which is approximately five times the recommended dose in humans, did significantly decrease the thrombus weight 90 and 180 min after the drug administration. In conclusion, sodium ibuprofen was shown to have an inhibitory effect on platelet function in vivo and in large doses was also found to diminish the thrombus weight.

Keywords

Ibuprofen - Platelets - Hemostasis - Fibrinolysis
 

  • References

  • 1 O’Brien JR. Effect of anti-inflammatory agents on platelets. Lancet 1968; 1: 894-895
    PubMedGoogle Scholar
  • 2 Baele G, De Weerdt GA, Barbier F. Preliminary results of platelet aggregation before and after administration of ibuprofen. Rheumatol Phys Med 1970; (Suppl. 10) 108-114
    PubMedGoogle Scholar
  • 3 Latini R, Villa S, Gerna M, Tognoni G, de Gaetano G. Bioavailability and platelet aggregation inhibitory activity of solufenum. A comparison with ibuprofen. Int J Clin Pharmacol 1977; 15: 492-495
    PubMedGoogle Scholar
  • 4 McIntyre BA, Philp RB. Effect of three nonsteroidal antiinflammatory agents on platelet function and prostaglandin synthesis in vitro. Thromb Res 1977; 12: 67-77
    PubMedGoogle Scholar
  • 5 Carson S, Demling R, Esquivel C, Talken L, Tillman P. Testing and treatment of arterial graft thrombosis. Am J Surg 1981; 142: 137-143
    CrossrefPubMedGoogle Scholar
  • 6 Chandler AB. In vitro thrombotic coagulation of the blood. A method for producing a thrombus. Lab Invest 1958; 7: 110-114
    PubMedGoogle Scholar
  • 7 Bergqvist D. Haemostatic plug formation in the rabbit mesentery. A methodological study. Ups J med Sci 1974; 79: 28-38
    CrossrefPubMedGoogle Scholar
  • 8 Arfors K-E, Jonsson JA, McKenzie FN. A titanium rabbit ear chamber: Assembly, insertion and results. Microvasc Res 1970; 2: 516-519
    CrossrefPubMedGoogle Scholar
  • 9 Arfors K-E, Hint HC, Dhall DP, Matheson NA. Counteraction of platelet activity at sites of laser-induced endothelial trauma. Br Med J 1968; 4: 430-431
    CrossrefPubMedGoogle Scholar
  • 10 Åberg M, Hedner U, Bergentz S-E. Effect of dextran on the lysability of ex vivo thrombi. Ann Surg 1975; 181: 342-345
    CrossrefPubMedGoogle Scholar
  • 11 Jacobsen CD, Chandler AB. Thrombolysis in vitro. I. Method comparison of various thrombolytic agents, and factors influencing thrombolysis. Scand J Clin Lab Invest 1965; 17: 209-224
    CrossrefPubMedGoogle Scholar
  • 12 Olson PS, Ljungqvist U, Bergentz S-E. Analysis of platelet red cell and fibrin content in experimental arterial and venous thrombi. Thromb Res 1974; 5: 1-19
    CrossrefPubMedGoogle Scholar
  • 13 Barnett HJM. A randomized trial of aspirin and sulfinpyrazone in threatened stroke. N Engl J Med 1978; 299: 53-59
    CrossrefPubMedGoogle Scholar
  • 14 Fields WS, Lemak NA, Frankowski RF, Hardy RJ. Controlled trial of aspirin in cerebral ischemia. Stroke 1977; 8: 301-306
    CrossrefPubMedGoogle Scholar
  • 15 McEnany MT, Salzman EW, Mundth ED, De Sanctis RW, Harthome JW, Weintraub RM, Gates S, Austen G. The effect of antithrombotic therapy on patency rates of saphenous vein coronary artery bypass grafts. J Thorac Cardiovasc Surg 1982; 83: 81-89
    PubMedGoogle Scholar
  • 16 Oblath RW, Buckler FO, Green RM, Schwartz SI, De Weese JA. Prevention of platelet aggregation and adherence to prosthetic vascular grafts by aspirin and dipyridamole. Surgery 1978; 84: 37-44
    PubMedGoogle Scholar
  • 17 Wieslander JB, Åberg M, Dougan P. Accumulation of isotope labelled platelets in small arteries after end-to-end and end-in-end anastomoses in the rabbit. Br J Plast Surg. In press
    PubMedGoogle Scholar
  • 18 Bergqvist D, Arfors KE. Influence of platelet count on hemostatic plug formation and plug stability. An experimental study in rabbits with graded thrombocytopenia. Thrombos Diathes Haemorrh 1973; 30: 586-596
    PubMedGoogle Scholar
  • 19 Bergqvist D, McKenzie FN, Arfors KE. Influence of fibrinolysis and coagulation in haemostatic plug formation. An experimental study in rabbits. Thromb Res 1975; 4: 345-360
    PubMedGoogle Scholar
  • 20 Bergqvist D, Arfors KE. Red blood cells and initial haemostatic plug formation. Microvasc Res (letter) 1976; 12: 237-238
    CrossrefPubMedGoogle Scholar
  • 21 McKenzie FN, Arfors KE, Matheson NA. Measurement of the platelet response to laser induced microvascular injury. Assessment of determinants of platelet aggregation in vivo. Thrombos Diathes Haemorrh 1974; 32: 704-713
    PubMedGoogle Scholar
  • 22 Hovig T, McKenzie FN, Arfors KE. Measurement of the platelet response to laser-induced microvascular injury. Ultrastructural studies. Thrombos Diathes Haemorrh 1974; 32: 695-703
    PubMedGoogle Scholar
  • 23 Szczeklik A, Gryglewski RJ, Czeniawska-Mysik G, Zmuda A. Aspirin-induced asthma hypersensitivity to fenoprofen and ibuprofen in relation to their inhibitory action on prostaglandin generation by different microsomal enzymic preparations. J Allergy Clin Immunol 1976; 58: 10-18
    CrossrefPubMedGoogle Scholar
  • 24 Rome LH, Lands WE. Structural requirements for time-dependent inhibition of prostaglandin biosynthesis by anti-inflammatory drugs. Proc Natl Acad Sci USA 1975; 72: 4863-4865
    CrossrefPubMedGoogle Scholar
  • 25 McIntyre BA, Philp RB, Inwood MJ. Effect of ibuprofen on platelet function in normal subjects and hemophiliacs. XII Cong. World Fed. Hemoph. Thromb Haemostas 1977; 38: 377 (Abstr.)
    PubMedGoogle Scholar
  • 26 Åberg M, Rausing A. The effect of Dextran 70 on the structure of ex vivo thrombi. Thromb Res 1978; 12: 1113-1122
    CrossrefPubMedGoogle Scholar
  • 27 Åberg M, Hedner U, Bergentz S-E. Effect of dextran on factor VIII (antihemophilic factor) and platelet function. Ann Surg 1979; 189: 243
    CrossrefPubMedGoogle Scholar
  • 28 Chalmers TM. Clinical experience with ibuprofen in the treatment of rheumatoid arthritis. Ann Rheum Dis 1969; 28: 513-517
    CrossrefPubMedGoogle Scholar
  • 29 Dick-Smith JB. Ibuprofen, Aspirin and Placebo in the treatment of rheumatoid arthritis – A double blind clinical trial. Med J Aust 1969; 2: 853-859
    PubMedGoogle Scholar
  • 30 Huskisson EC, Hart FD, Shenfield GM, Taylor RT. Ibuprofen. A review. Practitioner 1971; 207: 639-643
    PubMedGoogle Scholar