Subscribe to RSS
DOI: 10.1055/s-0038-1657277
A Simple, Sensitive Spectrophotometric Assay for Extrinsic (Tissue-Type) Plasminogen Activator Applicable to Measurements in Plasma
Publication History
Received 19 July 1982
Accepted 14 September 1982
Publication Date:
13 July 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/198203/lookinside/thumbnails/10-1055-s-0038-1657277-1.jpg)
Summary
An indirect spectrophotometric assay for extrinsic plasminogen activator has been devised, which is based on the parabolic assay of Drapier et al. (5). The system contains activator, plasminogen, the synthetic plasmin substrate H-D-Val-Leu-Lys-pNA (S-2251, Kabi) and a mixture of soluble fibrinogen fragments prepared by treatment of fibrinogen with cyanogen bromide. The addition of these fibrinogen fragments considerably enhances the sensitivity and specificity of the method owing to specific stimulation of the plasminogen activation by extrinsic plasminogen activator.
The assay conditions were optimized and the application for extrinsic plasminogen activator measurements in plasma euglobulin fractions is demonstrated.
-
References
- 1 Collen D. On the regulation and control of fibrinolysis. Thromb Haemosta 1980; 43: 77-89
- 2 Weimar W, Stibbe J, van Seyen AJ, Billiau A, De Somer P, Collen D. Specific lysis of an iliofemoral thrombus by administration of extrinsic (tissue-type) plasminogen activator. Lancet 1981; 2: 1018-1020
- 3 Kluft C. C1-inactivator-resistant fibrinolytic activity in plasma euglobulin fractions: Its relation to vascular activator in blood and its role ii euglobulin fibrinolysis. Thromb Res 1978; 13: 135-151
- 4 Kluft C. Quantitation and behaviour of extrinsic or vascular plasminoger activator in blood: Progress in Fibrinolysis. Davidson JF, Nilsson IM, Åstedt B. (Eds) Churchill Livingstone; Edinburgh: 1981. 5 24-30
- 5 Drapier IC, Tenue IP, Lemaire G, Petit IF. Regulation of plasminoger activator secretion in mouse peritoneal macrophages. Biochimie 1979; 61: 463-471
- 6 Rånby M, Wallén P. A sensitive parabolic rate assay for the tissue plasminoger activator. Progress in Fibrinolysis. Davidson JF, Nilsson IM, Åstedt B. (Eds) Churchill Livingstone; Edinburgh: 1981. 5: 233-235
- 7 Deutsch DG, Mertz ET. Plasminogen purification from human plasma by affinity chromatography. Science 1970; 170: 1095-1096
- 8 Rijken DC, Wijngaards G, Zaal-de JongM, Welbergen J. Purification and partial characterization of plasminogen activator from human uterine tissue. Biochim Biophys Acta 1979; 580: 140-153
- 9 Brommer EJP, Gevers Leuven JA, Barrett-Bergshoeff MM, Schouten JA. Response of fibrinolytic activity and factor VIII-related antigen to stimulation with desmopressin in hyperlipoproteinaemia. J Lab Clin Med 1982; 100: 105-114
- 10 Rijken DC, Wijngaards G, Welbergen J. Relationship between tissue plasminogen activator and the activators in blood and vascular wall. Thromb Res 1980; 18: 815-830
- 11 Verheijen JH, Nieuwenhuizen W, Wijngaards G. Activation of plasminogen by tissue activator is increased specifically in the presence of certain soluble fibrin(ogen) fragments. Thromb Res. 1982 in press
- 12 Haverkate F, Traas DW. Dose-response curves in the fibrin plate assay. Fibrinolytic activity of proteases. Thromb Haemostas 1974; 32: 356-365