Thromb Haemost 1982; 48(03): 266-269
DOI: 10.1055/s-0038-1657277
Original Article
Schattauer GmbH Stuttgart

A Simple, Sensitive Spectrophotometric Assay for Extrinsic (Tissue-Type) Plasminogen Activator Applicable to Measurements in Plasma

J H Verheijen
The Gaubius Institute, Health Research Division TNO, Leiden, The Netherlands
,
E Mullaart
The Gaubius Institute, Health Research Division TNO, Leiden, The Netherlands
,
G T G Chang
The Gaubius Institute, Health Research Division TNO, Leiden, The Netherlands
,
C Kluft
The Gaubius Institute, Health Research Division TNO, Leiden, The Netherlands
,
G Wijngaards
The Gaubius Institute, Health Research Division TNO, Leiden, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 19 July 1982

Accepted 14 September 1982

Publication Date:
13 July 2018 (online)

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Summary

An indirect spectrophotometric assay for extrinsic plasminogen activator has been devised, which is based on the parabolic assay of Drapier et al. (5). The system contains activator, plasminogen, the synthetic plasmin substrate H-D-Val-Leu-Lys-pNA (S-2251, Kabi) and a mixture of soluble fibrinogen fragments prepared by treatment of fibrinogen with cyanogen bromide. The addition of these fibrinogen fragments considerably enhances the sensitivity and specificity of the method owing to specific stimulation of the plasminogen activation by extrinsic plasminogen activator.

The assay conditions were optimized and the application for extrinsic plasminogen activator measurements in plasma euglobulin fractions is demonstrated.