Isolation of Human Fibrinogen of High Purity and in High Yield Using Polyethylene Glycol 1000

Marwan A Masri; Saad A Masri; Nigel D Boyd

doi:10.1055/s-0038-1657334

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1983; 49(02): 116-119
DOI: 10.1055/s-0038-1657334

Original Article

Schattauer GmbH Stuttgart

Isolation of Human Fibrinogen of High Purity and in High Yield Using Polyethylene Glycol 1000

Marwan A Masri

The Department of Medical Biochemistry, University of Calgary, Calgary, Alberta, Canada and Department of Genetics/Plant Science University of Alberta, Edmonton, Alberta, Canada

,

Saad A Masri

The Department of Medical Biochemistry, University of Calgary, Calgary, Alberta, Canada and Department of Genetics/Plant Science University of Alberta, Edmonton, Alberta, Canada

,

Nigel D Boyd

The Department of Medical Biochemistry, University of Calgary, Calgary, Alberta, Canada and Department of Genetics/Plant Science University of Alberta, Edmonton, Alberta, Canada

› Author Affiliations

Abstract

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Summary

Fibrinogen was isolated from human plasma using a polyethylene glycol 1000 fractionation procedure that eliminates problems of denaturation, degradation and contamination encountered with other procedures (1). The technique is simple, rapid, gives a high yield and gram amounts of fibrinogen can be readily obtained.

Keywords

Human fibrinogen - Isolation - Polyethylene glycol

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References

References
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