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Thromb Haemost 1983; 49(02): 116-119
DOI: 10.1055/s-0038-1657334
Original Article
Schattauer GmbH Stuttgart

Isolation of Human Fibrinogen of High Purity and in High Yield Using Polyethylene Glycol 1000

Marwan A Masri
The Department of Medical Biochemistry, University of Calgary, Calgary, Alberta, Canada and Department of Genetics/Plant Science University of Alberta, Edmonton, Alberta, Canada
,
Saad A Masri
The Department of Medical Biochemistry, University of Calgary, Calgary, Alberta, Canada and Department of Genetics/Plant Science University of Alberta, Edmonton, Alberta, Canada
,
Nigel D Boyd
The Department of Medical Biochemistry, University of Calgary, Calgary, Alberta, Canada and Department of Genetics/Plant Science University of Alberta, Edmonton, Alberta, Canada
› Author Affiliations
Further Information

Publication History

Received 08 July 1982

Accepted 07 February 1983

Publication Date:
26 July 2018 (online)

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Summary

Fibrinogen was isolated from human plasma using a polyethylene glycol 1000 fractionation procedure that eliminates problems of denaturation, degradation and contamination encountered with other procedures (1). The technique is simple, rapid, gives a high yield and gram amounts of fibrinogen can be readily obtained.

Keywords

Human fibrinogen - Isolation - Polyethylene glycol
 

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