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Thromb Haemost 1983; 49(03): 176-181
DOI: 10.1055/s-0038-1657356
Original Article
Schattauer GmbH Stuttgart

Effect of an Impermeant Arginine-Modifying Reagent on the Responses of Rabbit Platelets to Agonists

Esther R Anderson
The Department of Pharmacology, Faculty of Medicine, The University of British Columbia, Vancouver, Canada
,
Yum-Kin Au-Young
The Department of Pharmacology, Faculty of Medicine, The University of British Columbia, Vancouver, Canada
,
James G Foulks
The Department of Pharmacology, Faculty of Medicine, The University of British Columbia, Vancouver, Canada
› Author Affiliations
Further Information

Publication History

Received 23 November 1982

Accepted 22 March 1983

Publication Date:
26 July 2018 (online)

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Summary

The importance of surface arginyl residues in platelet aggregation was investigated by studying the effects of an impermeant arginine-modifying reagent, p-sulfonylphenylglyoxal (PSPG), on platelet responses to various agonists. Pretreatment of resuspended rabbit platelets with 2-15 mM PSPG resulted in complete inhibition of aggregation responses to ADP and 5-HT, and a concentration-dependent inhibition of the preceding shape change. Aggregation responses to thrombin also were inhibited in a concentration-dependent manner. The protective effects of antagonists of these three agonists (P, y-methylene ATP forADP, hirudin for thrombin and phentolamine for 5-HT) during pretreatment of platelets with PSPG indicated that intact arginine residues form part of the receptor sites for ADP and for thrombin. Arginine residues are not part of the 5-HT receptor site itself, but seem to be important for the maintenance of the functional integrity of this site.

Keywords

Platelets - Aggregation - Arginine-modification
 

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