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Thromb Haemost 1983; 49(03): 214-216
DOI: 10.1055/s-0038-1657365
Original Article
Schattauer GmbH Stuttgart

Lipid Peroxidation and Experimental Disseminated Intravascular Coagulation in Rats Induced by Endotoxin

T Yoshikawa
The First Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
M Murakami
The First Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
Y Furukawa
The First Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
H Kato
The First Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
S Takemura
The First Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
,
M Kondo
The First Department of Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan
› Author Affiliations
Further Information

Publication History

Received 16 October 1982

Accepted 03 March 1983

Publication Date:
18 July 2018 (online)

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Summary

The experimental model of disseminated intravascular coagulation (DIC) in rats induced by a 4 hr sustained infusion of 100 mg/kg endotoxin was used to study the relationship between lipid peroxidation and DIC. Serum thiobarbituric acid (TBA) reactive substances, important and damaging products of lipid peroxidation, increased gradually during the infusion of endotoxin. The levels of TBA reactants in serum, abdominal aortic wall and ileum mucosa were significantly increased in rats infused with 100 mg/kg of endotoxin for 4 hr, as compared with the control rats continuously given 11.4 ml of physiological saline for 4 hr. However, the levels in liver and kidney tissues, and in gastric, jejunum and colon mucosa did not increase significantly.

Keywords

DIC - Thiobarbituric acid reactive substances - Rat - Endotoxin - Lipid peroxidation
 

  • References

  • 1 Yoshikawa T, Furukawa Y, Murakami M, Watanabe K, Kondo M. Effect of vitamin E on endotoxin-induced disseminated intravascular coagulation in rats. Thromb Haemostas 1982; 48: 235-237
    PubMedGoogle Scholar
  • 2 Higashi O, Kikuchi Y. Effects of vitamin E on the aggregation and the lipid peroxidation of platelets exposed to hydrogen peroxide. Tohoku J Exp Med 1974; 112: 271-278
    CrossrefPubMedGoogle Scholar
  • 3 Tappel AL. Lipid peroxidation damage to cell components. Fed Proc 1973; 32: 1870-1874
    PubMedGoogle Scholar
  • 4 Minna ID, Robboy SI, Colman RW. Disseminated intravascular coagulation in man. Charles CThomas. (Ed) Springfield: 1974
    Google Scholar
  • 5 Beller FK, Graeff H. Deposition of glomerular fibrin in the rabbit after infusion with endotoxin. Nature 1967; 215: 295-296
    CrossrefPubMedGoogle Scholar
  • 6 Beller FK, Graeff H, Gorstein F. Disseminated intravascular coagulation during continuous infusion of endotoxin in rabbits: morphological and physiological studies. Am J Obstet Gynecol 1969; 103: 544-554
    CrossrefPubMedGoogle Scholar
  • 7 Yoshikawa T, Furukawa Y, Murakami M, Takemura S, Kondo M. Experimental model of disseminated intravascular coagulation induced by sustained infusion of endotoxin. Res Exp Med 1981; 179: 223-228
    CrossrefPubMedGoogle Scholar
  • 8 Dahle LK, Hill EG, Holman RT. The thiobarbituric acid reaction and the autoxidations of polyunsaturated fatty acid methyl esters. Arch Biochem 1962; 98: 253-261
    CrossrefPubMedGoogle Scholar
  • 9 Baker N, Wilson L. Water-soluble products of UV-irradiated autox-idized linoleic and linolenic acids. J Lipid Res 1966; 07: 341-348
    PubMedGoogle Scholar
  • 10 Pryor WA, Stanley JP. A suggested mechanism for the production of malonaldehyde during the autoxidation of polyunsaturated fatty acids. Nonenzymatic production of prostaglandin endoperoxides during autoxidation. J Organ Chem 1975; 40: 3615-3617
    CrossrefPubMedGoogle Scholar
  • 11 Ferreira HC, Murat LG. Immunological method for demonstrating fibrin degradation products in serum and its use the diagnosis of fibrinolytic states. Br J Haematol 1963; 09: 299-310
    CrossrefPubMedGoogle Scholar
  • 12 Goodwin JF. Estimation of plasma fibrinogen using sodium sulfate fractionation. Am J Clin Pathol 1961; 35: 227-232
    CrossrefPubMedGoogle Scholar
  • 13 Quick AJ, Stanly BM, Bancroft FW. A study of the coagulation defect in hemophilic and in jaundice. Am J Med Sci 1935; 190: 501-511
    CrossrefPubMedGoogle Scholar
  • 14 Langdell RD, Wager RH, Brinkhous KM. Effect of antihemophilic factor on one-stage clotting time. J Lab Clin Med 1953; 47: 637-647
    PubMedGoogle Scholar
  • 15 Brecker G, Cronkite EP. Morphology and enumeration of human blood platelets. J Appl Physiol 1950; 03: 365-377
    CrossrefPubMedGoogle Scholar
  • 16 Weigert C. Über eine neue Methode zur Färbung von Fibrin und von Mikroorganismen. Fortsch Med 1887; 05: 228-232
    PubMedGoogle Scholar
  • 17 Yagi K. A simple fluorometric assay for lipid peroxide in blood plasma. Biochem Med 1976; 15: 212-216
    CrossrefPubMedGoogle Scholar
  • 18 Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxide for animal tissues by thiobarbituric add reaction. Anal Biochem 1979; 95: 351-358
    CrossrefPubMedGoogle Scholar
  • 19 Moncada S, Vane JR. Unstable metabolites of arachidonic acid and their role in hemostasis and thrombosis. Br Med Bull 1978; 34: 129
    CrossrefPubMedGoogle Scholar
  • 20 Moncada S, Higgs EA, Vane JR. Human arterial and venous tissues generate prostacyclin (prostaglandin X), a potent inhibitor of platelet aggregation. Lancet 1977; 01: 18
    PubMedGoogle Scholar
  • 21 Granger DN, Rutili G, McCord JM. Superoxide radicals in feline intestinal ischemia. Gastroenterology 1981; 81: 22-29
    PubMedGoogle Scholar
  • 22 Parks DA, Bulkley GB, Granger DN, Hamilton SR, McCord JM. Ischemic injury in the cat small intestine: role of superoxide radicals. Gastroenterology 1982; 82: 09-15
    PubMedGoogle Scholar