The Regulation of Platelet Aggregation In Vitro by Interleukin-1 β and Tumor Necrosis Factor-α: Changes in Pregnancy and in Pre-eclampsia

J Bar; A Zosmer; M Hod; M G Elder; M H F Sullivan

doi:10.1055/s-0038-1657724

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1997; 78(04): 1255-1261
DOI: 10.1055/s-0038-1657724

Rapid Communication

Schattauer GmbH Stuttgart

The Regulation of Platelet Aggregation In Vitro by Interleukin-1 β and Tumor Necrosis Factor-α: Changes in Pregnancy and in Pre-eclampsia

J Bar

¹The Department of Obstetrics and Gynaecology, Rabin Medical Centre, Petah Tiqva and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

,

A Zosmer

²The R.P.M.S. Institute of Obstetrics and Gynaecology, Hammersmith Hospital, London, UK

,

M Hod

¹The Department of Obstetrics and Gynaecology, Rabin Medical Centre, Petah Tiqva and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

,

M G Elder

²The R.P.M.S. Institute of Obstetrics and Gynaecology, Hammersmith Hospital, London, UK

,

M H F Sullivan

²The R.P.M.S. Institute of Obstetrics and Gynaecology, Hammersmith Hospital, London, UK

› Author Affiliations

Abstract

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Summary

Platelet activation occurs in early pregnancy in women at risk of developing pre-eclampsia. Cytokines have been implicated in the pathogenesis of pre-eclampsia, so we determined the effects of interleukin- 1β (IL-1β) and tumor necrosis factor-α (TNF-α) on the in vitro aggregation of human platelets. IL-1β increased aggregation of platelets from non-pregnant and pre-eclamptic women, and inhibited the aggregation of platelets from normal pregnant women. This latter effect was linked to a diminished P-selectin expression on ADP-stimulated whole blood platelets in normal pregnant women (p = 0.011). Platelet aggregation in response to ADP was found to be inhibited after preincubation with TNF-α in non-pregnant (38%, p = 0.01) and in normal pregnant women (54%, p = 0.001) and not affected in pre-eclamptic women. The inhibitory effects of TNF-α were mediated through the P75 receptor for TNF-α.

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References

References
1 Hall MH, Chang PK, McGillivray I. Is routine antenatal care worth while?. Lancet 1980; ii: 78-80
2 Lyall F, Greer IA. Preeclampsia: a multifaceted vascular disorder of pregnancy. J Hypertens 1994; 12: 1339-1345
3 McCarthy AL, Woolfson RG, Raju SK, Poston L. Abnormal endothelial cell function of resistance arteries from women with pre-eclampsia. Am J Obstet Gynecol 1993; 168: 1323-1330
4 Fitzgerald DJ. Decreased prostacyclin biosynthesis preceded the onset of clinical manifestation of pregnancy-induced hypertension. Circulation 1987; 75: 956-963
5 Redman CWG. Platelets and the beginning of pre-eclampsia. Lancet 1990; ii: 478-480
6 Socol ML. Platelet activation in pre-eclampsia. Am J Obstet Gynecol 1985; 151: 494-497
7 Rakoczi I, Tallian F, Bagdany S, Gati I. Platelet life-span in normal pregnancy and pre-eclampsia as determined by non-radioisotope technique. ThrombRes 1979; 15: 553-556
8 Giles C, Inglis TCM. Thrombocytopenia and macrothrombocytosis in gestational hypertension. Br J Obstet Gynaecol 1981; 88: 1115-1119
9 Whigham KA, Howie PW, Drummond HA, Prentice CR. Abnormal platelet function in pre-eclampsia. Br J Obstet Gynaecol 1978; 85: 28-32
10 DeWolf F, DeWolf-Peeters C, Brosens I, Robertson WB. The human placental bed: Electron microscopic study of trophoblastic invasion of spiral arteries. Am J Obstet Gynecol 1980; 137: 58-70
11 Pijnenborg R, Bland JM, Robertson WB, Brosens I. Uteroplacental arterial changes related to interstitial trophoblast migration in early human pregnancy. Placenta 1983; 4: 397-414
12 Robertson WB. Uteroplacental vasculature. J Clin Pathol (Suppl) 1976; 10: 09-17
13 Zemel MB, Zemel PC, Berry S, Norman G, Kowalczyk C, Sokol RJ, Standley PR, Walsh MF, Sowers JR. Altered platelet calcium metabolism as an early predictor of increased peripheral vascular resistance and pre-eclampsia in urban black women. New Engl J Med 1990; 323: 434-438
14 Sullivan MHF, Johnson S, Elder MG. Platelet malfunction preceding the onset of pregnancy induced hypertension. J Obstet Gynaecol 1990; 10: 215-216
15 Janes SL, Kyle PM, Redman C, Goodall AH. Flow cytometry detection of activated platelets in pregnant women prior to the development of pre-eclampsia. Thromb Haemost 1995; 74: 1059-1063
16 Scott JR, Beer AA. Immunological aspects of pre-eclampsia. Am J Obstet Gynecol 1976; 125: 418-427
17 Redman CWG. Immunological aspects of pre-eclampsia. Bailliere’s Clin Obstet Gynecol 1992; 6: 601-615
18 Wood GW, De M, Sanford TH. Production of interleukin 1, interleukin 6 and tumor necrosis factor alpha in response to estrogen and progesterone during the periimplantation period of pregnancy. J Cell Biochem 1992; 16 B: 291
19 Hunt JS. Current topics: The role of macrophage in the uterine response to pregnancy. Placenta 1990; 11: 467-475
20 Hill JA. 1992 Cytokines considered critical in pregnancy. Am J Reprod Immunol. 28. 123-126
21 Lewis MP, Sullivan MHF, Elder MG. Regulation by Interleukin-1β of growth and collagenase production by choriocarcinoma cells. Placenta 1994; 15: 13-20
22 Kuperminc MJ, Peaceman AM, Wigton TR, Rehnberg KA, Socol ML. Tumor necrosis factor-α is elevated in plasma and amniotic fluid of patients with severe pre-eclampsia. Am J Obstet Gynecol 1994; 170: 1752-1759
23 Keith Jr JC, Rowles TK, DiLorenzo K, Mishra S, Mishra HP. Tumor necrosis factor-α may mediate maternal serum cytotoxicity during preeclampsia. J Perinatol 1993; 13: 417-418
24 Keith Jr JC, Pijnenborg R, Spitz B, Hanssens M, Van Assche FA. Assessment of differential serum cytotoxicity in gestational hypertension using a fibrosarcoma cell line and the MTT assay. Hypertens Preg 1995; 14: 81-90
25 Heybome KD, Witkin SS, McGregor JA. Tumor necrosis factor-α in midtrimester amniotic fluid is associated with impaired intrauterine fetal growth. Am J Obstet Gynecol 1992; 167: 920-925
26 Kuperminc MJ, Peaceman AM, Aderka D, Wallach D, Socol ML. Soluble tumor necrosis factor receptors and interleukin-6levels in patients with severe pre-eclampsia. Obstet Gynecol 1996; 88: 420-427
27 Radomski MW, Vallance P, Whitley G, Foxwell N, Moncada S. Platelet adhesion to human vascular endothelium modulated by constitutive and cytokine induced nitric oxide. Cardiovasc Res 1993; 27: 1380-1382
28 Harwrylowicz CM, Howells GL, Feldmann M. Platelet-derived interleukin-1 induces human endothelial adhesion molecule expression and cytokine production. J Exp Med 1991; 174: 785-790
29 Sedlmayr P, Blaschitz A, Wilders-Truschnig M, Tiran A, Dohr G. Platelets contain interleukin-la and (3 which are detectable on the cell surface after activation. Scand J Immunol 1995; 42: 209-214
30 Mehta JL, Chen LY, Kone BC, Mehta P, Turner P. Identification of constitutive and inducible forms of nitric oxide synthase in human platelets. J Lab Clin Med 1995; 125: 370-377
31 Radomski MW, Palmer RMJ, Moncada S. The role of nitric oxide and cGMP in platelet adhesion to vascular endothelium. Biochem Biophys Res Commun 1987; 148: 1482-1489
32 Habrugge M, Walter U. 1993. In: Protein kinases in blood cell function. Huang CK, Sha’afi RI. eds. CRC Press; Boca Raton: 1993. pp 245-298
33 Redman CWG, Jefferies M. Revised definition of pre-eclampsia. Lancet 1988; i: 809-812
34 Brockhaus M, Schoenfeld HJ, Schlaeger EJ, Hunziker W, Lesslauer W, Loetscher H. Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies. Proc Natl Acad Sci USA 1990; 87: 3127-3131
35 Van-Ostade X, Vandenbeele P, Tavemier J, Fiers W. Human tumor necrosis factor mutants with preferential binding activity on either the R55 or R75 receptor. Eur J Biochem 1994; 220: 771-779
36 Bom GVR, Cross MJ. The aggregation of blood platelets. J Physiol 1963; 168: 178-195
37 Schaufelberger HD, Uhr MR, McGuckin C, Logan RP, Misiewicz JJ, Gordon-Smith EC, Beglinger C. 1994. Platelets in ulcerative colitis and Crohn’s disease express functional interleukin-1 and interleukin-8 receptors. Eur J Clin Invest 1994; 24: 656-663
38 Todoroki N, Watanabe Y, Akaike T. Enhancement by IL-1β and IFN γ of platelet activation: Adhesion of leukocytes via GMP-140/PADGEM protein (CD62). Biochem Biophys Res Commun 1991; 179: 756-761
39 Uhr MR, Schaufelberger HD, Hildbrand P, Beglinger C. 1995. Thrombocytes express functional cytokine receptors in patients with Crohn disease. Schweiz Ned Wochenschr 1995; 125: 970-974
40 Cole OF, Seki H, Elder MG, Sullivan MHF. Interleukin-1β independently stimulates production of prostaglandin E₂and cyclic AMP from human decidual cells. Biochim Biophys Acta 1995; 1269: 139-144
41 Huleihel M, Leiberman JR, Yohay D, Glezerman M. Decreased interleukin-6 production by rat uterine artery, aorta and uterine tissues during pregnancy. Human Reprod 1996; 11: 1337-1342
42 Hohmann HPR, Remy R, Brockhaus M, van Loon APGM. Two different cell types have different major receptors for human tumor necrosis factor (TNF-α). J Biol Chem 1989; 264: 14927-14934
43 Gant NF, Whalley PJ, Everett RB, Worley RJ, MacDonald PC. Control of vascular reactivity in pregnancy. Am J Kidney Dis 1987; 9: 303-307
44 Gordon MS, Hoffman R. Growth factors affecting human thrombocyto- poiesis: potential agents for the treatment of thrombocytopenia. Blood 1992; 80: 302-307
45 Janes SL, Goodall AH. Row cytometry detection of circulating activated platelets and platelet hyper-responsiveness in pre-eclampsia and pregnancy. Clin Sci 1994; 86: 731-739