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Thromb Haemost 1997; 78(04): 1255-1261
DOI: 10.1055/s-0038-1657724
Rapid Communication
Schattauer GmbH Stuttgart

The Regulation of Platelet Aggregation In Vitro by Interleukin-1 β and Tumor Necrosis Factor-α: Changes in Pregnancy and in Pre-eclampsia

J Bar
1   The Department of Obstetrics and Gynaecology, Rabin Medical Centre, Petah Tiqva and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
,
A Zosmer
2   The R.P.M.S. Institute of Obstetrics and Gynaecology, Hammersmith Hospital, London, UK
,
M Hod
1   The Department of Obstetrics and Gynaecology, Rabin Medical Centre, Petah Tiqva and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
,
M G Elder
2   The R.P.M.S. Institute of Obstetrics and Gynaecology, Hammersmith Hospital, London, UK
,
M H F Sullivan
2   The R.P.M.S. Institute of Obstetrics and Gynaecology, Hammersmith Hospital, London, UK
› Author Affiliations
Further Information

Publication History

Received 17 1997

Accepted after resubmission 24 June 1997

Publication Date:
12 July 2018 (online)

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Summary

Platelet activation occurs in early pregnancy in women at risk of developing pre-eclampsia. Cytokines have been implicated in the pathogenesis of pre-eclampsia, so we determined the effects of interleukin- 1β (IL-1β) and tumor necrosis factor-α (TNF-α) on the in vitro aggregation of human platelets. IL-1β increased aggregation of platelets from non-pregnant and pre-eclamptic women, and inhibited the aggregation of platelets from normal pregnant women. This latter effect was linked to a diminished P-selectin expression on ADP-stimulated whole blood platelets in normal pregnant women (p = 0.011). Platelet aggregation in response to ADP was found to be inhibited after preincubation with TNF-α in non-pregnant (38%, p = 0.01) and in normal pregnant women (54%, p = 0.001) and not affected in pre-eclamptic women. The inhibitory effects of TNF-α were mediated through the P75 receptor for TNF-α.

 

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