Endothelial Cells Degrade Extracellular Matrix Proteins Produced In Vitro

Walter E Laug; Mark E Weinblatt; Peter A Jones

doi:10.1055/s-0038-1657882

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1985; 54(02): 498-502
DOI: 10.1055/s-0038-1657882

Original Article

Schattauer GmbH Stuttgart

Endothelial Cells Degrade Extracellular Matrix Proteins Produced In Vitro

Authors

Walter E Laug

The Division of Hematology-Oncology, Childrens Hospital of Los Angeles and University of Southern California, School of Medicine, Los Angeles, California, USA
Mark E Weinblatt

The Division of Hematology-Oncology, Childrens Hospital of Los Angeles and University of Southern California, School of Medicine, Los Angeles, California, USA
Peter A Jones

The Division of Hematology-Oncology, Childrens Hospital of Los Angeles and University of Southern California, School of Medicine, Los Angeles, California, USA

Abstract

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Summary

Bovine aortic endothelial cells (BAEC) were grown on extracellular matrices produced by vascular smooth muscle cells or fetal bovine endothelial cells. The glycoprotein components of these complex substrates were degraded through activation of the serum zymogen plasminogen to plasmin, as well as by a plasmino- gen independent protease(s). The plasminogen independent enzyme might be a protease with elastolytic activity since the BAEC digested elastin present in smooth muscle cell derived matrices. The cells also displayed collagenolytic activity on both types of matrices.

The addition of the tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate (TPA) (10⁻⁷ M) to the culture medium enhanced considerably the plasminogen activator and collagenolytic activities elaborated by BAEC resulting in an increased degradation rate of matrix glycoprotein and collagen components, whereas the elastolytic activity remained unaffected. Dex- amethasone (10⁻⁷ M), although suppressing plasminogen activator (PA) production by BAEC, did not alter their elastolytic activity allowing the cells to degrade the glycoprotein components of the matrices at an unchanged rate. The collagenolytic activity of the BAEC remained unaffected by dexamethasone.

These studies demonstrate that BAEC elaborate different proteolytic enzyme activities allowing them to degrade various components of extracellular matrices. These enzymatic activities may be modulated by certain agents thus changing the degradative capabilities of the BAEC.

Keywords

Endothelial Cells - Extracellular Matrix - Proteases - Matrix degradation -

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References

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