Plasma Concentrations of Platelet-Specific Proteins in Different Stages of Essential Hypertension: Interactions between Platelet Aggregation, Blood Lipids and Age

J Yamanishi; H Sano; K Saito; Y Furuta; H Fukuzaki

doi:10.1055/s-0038-1657892

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1985; 54(02): 539-543
DOI: 10.1055/s-0038-1657892

Original Article

Schattauer GmbH Stuttgart

Plasma Concentrations of Platelet-Specific Proteins in Different Stages of Essential Hypertension: Interactions between Platelet Aggregation, Blood Lipids and Age

J Yamanishi

The First Department of Internal Medicine, Kobe University School of Medicine, Kobe, Japan

,

H Sano

The First Department of Internal Medicine, Kobe University School of Medicine, Kobe, Japan

,

K Saito

The First Department of Internal Medicine, Kobe University School of Medicine, Kobe, Japan

,

Y Furuta

The First Department of Internal Medicine, Kobe University School of Medicine, Kobe, Japan

,

H Fukuzaki

The First Department of Internal Medicine, Kobe University School of Medicine, Kobe, Japan

› Institutsangaben

Abstract

als PDF herunterladen

Summary

Plasma β-thromboglobulin (βTG) and platelet factor 4 (PF4) were significantly higher in a group of 116 hypertensive men than in a normotensive group of 142 men. They increased with the stage of hypertension but the level did not correlate with the age of the subjects. Platelet aggregation was similar in the two groups and positively correlated with the age of the subjects in the normotensive group but not in the hypertensive group. A strong positive correlation was observed between the levels of plasma βTG and PF4 and between platelet aggregation to ADP and that to epinephrine in both the hypertensive and normotensive groups. However, there was no correlation between the level of plasma βTG or PF4 and platelet aggregation. Plasma antithrombin III was lower in the hypertensive group than in the normotensive group.

These studies suggest that plasma levels of PTG and PF4 are closely related to the stage of hypertension and are better indicators than aggregation of in vivo platelet activation in hypertensive subjects. Enhanced platelet activation may be involved in the acceleration of hypertensive arteriovascular damage and atherosclerosis.

Keywords

Platelet aggregation - Hypertension - Age - β-thromboglobulin - Platelet factor 4

PDF (1120 kb)

Referenzen

References
1 Young W, Gofman JW, Tandy R, Malamud N, Waters ESG. The quantitation of atherosclerosis, II. quantitative aspects of the relationship of blood pressure and atherosclerosis. Am J Cardiol 1960; 06: 294-299
2 Baker AB, Resch JA, Loewenson RB. Hypertension and cerebral atherosclerosis. Circulation 1969; 39: 701-710
3 Mustard JF, Murphy EA, Rowsell HC, Downie HG. Platelets and atherosclerosis. J Atheroscler Res 1964; 04: 01-28
4 Ross R, Glomset JA. The pathogenesis of atherosclerosis. N Engl J Med 1976; 295: 369-377 420
5 Steele PP, Weily HS, Davies H, Genton E. Platelet function studies in coronary artery disease. Circulation 1973; 48: 1194-1200
6 Acheson J, Danta G, Hutchinson EC. Platelet adhesiveness in patients with cerebral vascular disease. Atherosclerosis 1972; 15: 123-127
7 Johnson M, Ramey E, Ramwell PW. Sex and age differences in human platelet aggregation. Nature 1975; 253: 355-357
8 Yokoyama M, Kawashima S, Sakamoto S, Akita H, Okada T, Mizutani T, Fukuzaki H. Platelet reactivity and its dependence on alpha-adrenergic receptor function in patients with ischaemic heart disease. Br Heart J 1983; 49: 20-25
9 Carvalho ACA, Colman RW, Lees RS. Platelet function in hyperlipoproteinemia. N Engl J Med 1974; 290: 434-438
10 Kwaan HC, Colwell JA, Cruz S, Suwanwela N, Dobbie JG. Increased platelet aggregation in diabetes mellitus. J Lab Clin Med 1972; 80: 236-246
11 Poplawski A, Skorulska M, Niewiarowski S. Increased platelet adhesiveness in hypertensive cardiovascular disease. J Atheroscler Res 1968; 08: 721-723
12 Coccheri S, Fiorentini P. Platelet adhesiveness and aggregation in hypertensive patients. Acta Med Scand Suppl 1971; 525: 273-275
13 Vlachakis ND, Aledort FL. Hypertension and propranolol therapy: effect on blood pressure, plasma catecholamines and platelet aggregation. Am J Cardiol 1980; 45: 321-325
14 Kaplan KL. Platelet granule proteins: localization and secretion. In: Platelets in Biology and Pathology 2. Gordon JL. (Ed). Elsevier/North-Holland Biomedical Press; Amsterdam: 05 1981: 77-90
15 Smith RC, Duncanson J, Ruckley CV, Webber RG, Allan NC, Dawes J, Bolton AE, Hunter WM, Pepper DS, Cash JD. β-thromboglobulin and deep vein thrombosis. Thromb Haemostas 1978; 39: 338-345
16 Doyle DJ, Chesterman CN, Cade JF, McGready JR, Rennie GC, Morgan FJ. Plasma concentrations of platelet-specific proteins correlated with platelet survival. Blood 1980; 55: 82-84
17 Cella G, Zahavi J, de Haas HA, Kakkar VV. β-thromboglobulin, platelet production time and platelet function in vascular disease. Br J Haematol 1979; 43: 127-136
18 Born GVR. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature 1962; 194: 927-929
19 Ludlam CA, Moore S, Bolton AE, Pepper DS, Cash JD. The release of a human platelet specific protein measured by a radio immunoassay. Thromb Res 1975; 06: 543-548
20 Allain CC, Poon LS, Chan CSG, Richmond W, Fu PC. Enzymatic determination of total serum cholesterol. Clin Chem 1974; 20: 470-475
21 Eggstein M, Kreutz FH. Eine neue Bestimmung der Neutralfette in Blutserum und Gewebe. Klin Wochenschr 1966; 44: 262-267
22 Burstein M, Scholnick HR, Morfin R. Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. J Lipid Res 1970; 11: 583-595
23 Petralito A, Fiore CE, Mangiafico RA, Malatino LS. Beta-thromboglobulin plasma levels in different stages of arterial hypertension. Thromb Haemostas 1982; 48: 241
24 Mehta J, Mehta P. Platelet function in hypertension and effect of therapy. Am J Cardiol 1981; 47: 331-334
25 Andrassy K, Deppermann D, Ritz E, Koderisch J, Seeling H. Different effects of renal failure on beta-thromboglobulin and high affinity platelet factor 4 (HA-PF4)-concentrations. Thromb Res 1980; 18: 469-475
26 Torsellini A, Becucci A, Citi S, Cozzolino F, Guidi G, Lombaridi V, Vercelli D, Veloci M. Effects of pressure excursions on human platelets. In vitro studies on beta thromboglobulin (βTG) and platelet factor 4 (PF4) release and on platelet sensitivity to ADP-aggregation. Haematologica 1982; 67: 860-866
27 Bowie EJW, Owen Jr CA. Some factors influencing platelet retension in glass bead columns including the influence of plastics. Am J Clin Pathol 1971; 71 (Suppl. 56) 479-483
28 Yokoyama M, Kusui A, Sakamoto S, Fukuzaki H. Age-associated increments in human platelet α-adrenoreceptor capacity: possible mechanism for platelet hyperactivity to epinephrine in aging man. Thromb Res 1984; 34: 287-295
29 Folts JD, Crowell EB, Rowe GG. Platelet aggregation in partially obstructed vessels and its elimination with aspirin. Circulation 1976; 54: 365-370
30 Zahavi J, Betteridge JD, Jones NAG, Galton DJ, Kakkar VV. Enhanced in vivo platelet release reaction and malondialdehyde formation in patients with hyperlipidemia. Am J Med 1981; 70: 59-64
31 Sadoshima S, Kurozumi T, Tanaka K, Ueda K, Takeshita M, Hirota Y, Omae T, Uzawa H, Katsuki S. Cerebral and aortic atherosclerosis in Hisayama, Japan. Atherosclerosis 1980; 36: 117-126
32 Sano K, Takai Y, Yamanishi J, Nishizuka Y. A role of calcium-activated phospholipid-dependent protein kinase in human platelet activation. J Biol Chem 1983; 258: 2010-2013
33 Bick RL, Kovacs I, Fekete LF. A new two-stage functional assay for antithrombin-III (heparin cofactor): Clinical and laboratory evaluation. Thromb Res 1976; 08: 745-756
34 Bick RL, Dukes ML, Wilson WL, Fekete LF. Antithrombin III (AT-III) as a diagnostic aid in disseminated intravascular coagulation. Thromb Res 1977; 10: 721-729