Subscribe to RSS
DOI: 10.1055/s-0038-1660130
Thrombin Activity Appearing on the Vessel Wall After Trauma
Publication History
Received 11 December 1984
Accepted 21 August 1985
Publication Date:
19 July 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/198504/lookinside/thumbnails/10-1055-s-0038-1660130-1.jpg)
Summary
Thrombin activity was assayed on the aortic surface of rabbits after soft tissue trauma or endothelial injury caused by a balloon catheter. The animals were sacrificed by exsanguination 20 minutes or 3 hours after either type of trauma. Thrombin amidolytic activity on the luminal surface of the aorta was measured by exposing it to a synthetic chromogenic substrate.
Thrombin activity appeared on the aortic endothelium 3 hours but not 20 minutes after soft tissue trauma. Heparin prevented the appearance of thrombin activity completely only if given just before the trauma. After endothelial injury, thrombin activity appeared on the vascular surface after 3 hours but not after 20 minutes.
Thrombin activity appearing on the endothelium after soft tissue trauma may explain posttraumatic thrombotic events. The thrombin activity could, however, also be directed towards activation of protein C in which case an anticoagulant effect is obtained. Thrombin appearing after endothelial injury may enhance reactivity on the damaged vessel wall.
-
References
- 1 Bergentz SE, Nilsson IM. Effect of trauma on coagulation and fibrinolysis in dogs. Acta Chir Scand 1961; 122: 21-29
- 2 Nemerson Y, Pitlick FA. The tissue factor pathway of blood coagulation. In: Progress in Hemostasis and Thrombosis Spaet TH. Editor. Grune and Stratton, New York: 1972. 1 1-37
- 3 Griffin JH, Cochrane CG. Recent advances in the understanding of contact activation reactions. Semin Thromb Haemostas 1979; 5: 254-273
- 4 Niewiarowski S, Stuart RK, Thomas DP. Activation of intravascular coagulation by collagen. Proc Soc Exp Biol Med 1966; 123: 169-200
- 5 Holmer E, Söderberg G, Andersson LO. Studies on the mechanism of the rate-enhancing effect of heparin on the thrombin-antithrombin III reaction. Eur J Biochem 1979; 93: 1-5
- 6 Dryjski M, Olsson P, Swedenborg J. Uptake and inhibition of thrombin by the vascular wall. Thromb Res 1982; 27: 467-475
- 7 Lollar P, Owen WG. Clearance of thrombin from the circulation in rabbits by high affinity binding sites on endothelium. J Clin Invest 1980; 66: 1222-1230
- 8 Busch C, Owen WG. Identification in vitro of an endothelial cell surface co-factor for antithrombin III. J Clin Invest 1982; 69: 726-729
- 9 Teien A, Abildgaard U, Hook M. The anticoagulant effect of heparan sulphate and dermatan sulphate. Thromb Res 1976; 8: 859-867
- 10 Swedenborg J, Dryjski M, Olsson P. Inactivation of thrombin by aortic endothelium. Thromb Haemostas 1983; 50: 635-638
- 11 Jaffe EA, Hoyer LW, Nachman RL. Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J Clin Invest 1973; 52: 2757-2764
- 12 Loskutoff DJ, Edgington TD. Synthesis of a fibrinolytic activator and inhibitor by endothelial cells. Proc Natl Acad Sci USA 1977; 74: 3903-3907
- 13 Owen WG, Esmon CT. Functional properties of an endothelial cell co-factor for thrombin catalyzed activation of protein C. J Biol Chem 1981; 11: 5532-5535
- 14 Claesson G, Aurell L, Karlsson G, Gustavsson S, Friberger P, Arnelly S, Simonsson R. Design of chromogenic peptide substrates. In: Chromogenic peptide substrates: chemistry and clinical usage Scully MF, Kakkar V. (Eds). Churchill Livingstone: 1979: 20-31
- 15 Esmon CT, Esmon NL, Harris KW. Complex formation between thrombin and thrombomodulin inhibits both thrombin catalyzed fibrin formation and factor V activities. J Biol Chem 1982; 257: 7944-7947
- 16 Borgström S, Gelin LE, Zederfeldt B. The formation of vein thrombi following tissue injury. Acta Chir Scand 1959 Suppl. 247
- 17 Helin R, Lorenzon J, Garbarsch C, Matthiessen ME. Repair in arterial tissue. Circ Res 1971; 29: 542-554
- 18 Björkerud S, Bondjers G. Arterial repair and atherosclerosis after mechanical injury. Atherosclerosis 1971; 14: 259-276
- 19 Spaet TH, Stemerman MB, Veith FJ, Lejnieks J. Intimal injury and regrowth in the rabbit aorta. Circ Res 1975; 36: 58-70
- 20 Groves HM, Kinlough-Rathbone RL, Richardson M, Moore S, Mustard JF. Platelet interaction with damaged rabbit aorta. Lab Invest 1979; 40: 194-200
- 21 Essien EM, Cazenave JP, Moore S, Mustard JF. Effect of heparin and thrombin on platelet adherence to the surface of rabbit aorta. Thromb Res 1978; 13: 69-78
- 22 Czervionke RL, Hoak JC, Glenna LF. Effect of aspirin on thrombin-induced adherence of platelets to cultured cells from the blood vessel wall. J Clin Invest 1978; 62: 847-869
- 23 Hiebert LM, Jaques LB. The observation of heparin on endothelium after injection. Thromb Res 1976; 8: 195-204
- 24 Hatton MW C, Dejana E, Cazenave JP, Regoeczi E, Mustard JF. Heparin inhibits thrombin binding to rabbit thoracic aorta endothelium. J Lab Clin Med 1980; 96: 861-970