RSS-Feed abonnieren
Bitte kopieren Sie die angezeigte URL und fügen sie dann in Ihren RSS-Reader ein.
https://www.thieme-connect.de/rss/thieme/de/10.1055-s-00035024.xml
Thromb Haemost 1984; 51(01): 009-011
DOI: 10.1055/s-0038-1660997
DOI: 10.1055/s-0038-1660997
Original Article
Antithrombin III Determination by Rate (Kinetic) Nephelometry
Weitere Informationen
Publikationsverlauf
Received 12. Oktober 1982
Accepted 31. Oktober 1983
Publikationsdatum:
19. Juli 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/198401/lookinside/thumbnails/10-1055-s-0038-1660997-1.jpg)
Summary
Rate nephelometry immunological antithrombin III determination was elaborated with Beckman Immunochemistry System. The measuring can be carried out within 60 sec from 20 μl plasma. The results show a close correlation with those of gained by the radial immunodiffusion method (r = 0.91) and by Coatest (Kabi-Ortho) (r = 0.77). Both laser-nephelometric and usual non-nephelometric anti-antithrombin III serum (Behring) may be applied.
-
References
- 1 Bick RL. Clinical relevance of anti thrombin III. Semin Thromb Haemostas 1982; 8: 276-287
- 2 Fareed J, Messmore HL, Walenga JM, Bermes EW, Bick RL. Laboratory evaluation of antithrombin III: A critical overview of currently available methods for antithrombin III measurements. Semin Thromb Haemostas 1982; 8: 288-301
- 3 Fareed J, Messmore HL, Walenga JM, Bermes Jr EW. Diagnostic efficacy of newer synthetic-substrates methods for assessing coagulation variables: A critical overview. Clin Chem 1983; 29: 225-236
- 4 Mancini G, Carbonara AO, Hermans JF. Immunochemical quantitation of antigens by single radial immundiffusion. Int J Immunochem 1965; 2: 235-243
- 5 Messmore HL. Natural inhibitors of the coagulation system. Semin Thromb Haemostas 1982; 8: 267-275
- 6 Parvez Z, Fareed J, Messmore HL, Bermes EW. Laser and rate nephelometric methods for the quantitation of coagulation proteins. In: Seminar on Biochemical Hematology. Sunderman FW. (Ed.) Association of Clinical Scientists; Philadelphia: 1979. p 404
- 7 Parvez Z, Fareed J, Messmore HL, Moncada R. Laser nephelometric quantitation of antithrombin-III (AT-III) development of a new assay. Thromb Res 1981; 24: 267-377
- 8 Schmitz-Heubner U, Nachbar J, Asbeck F. The determination of antithrombin-III, α2-macroglobulin and α2-antiplasmin in plasma by laser nephelometry. J Clin Chem Clin Biochem 1980; 18: 221-226
- 9 Scully MF, Kakkar VV. Methods for semi-micro or automated determination of thrombin, antithrombin and heparin cofactor using the substrate H-D-Phe-Pip-Arg-P-Nitroanilide-2 HCL. Clin Chim Acta 1977; 79: 595-602
- 10 Sternberg JC. A rate nephelometer for measuring specific proteins by immunoprecipitin reactions. Clin Chem 1977; 23: 1456-1464
- 11 Whicher JT, Price CP, Spencer K. Immunonephelometric and immunoturbidometric assays for proteins. CRC Crit Rev Clin Lab Sri 1983; 18: 213-360
- 12 Wickerhauser M, Hao YL. Large-scale of plasma components for clinical use. In: Perspectives in hemostasis. Fareed J, Messmore HL, Fenton II JW, Brinkhous KM. (Eds) Pergamon Press Inc; New York: 1981. pp 258-268
- 13 Wider G, Kulnigg E, Molinari E, Hotschek H, Bayer PM. Evaluation of a monitor guided nephelometric system. J Clin Chem Clin Biochem 1982; 20: 1-8