A Study of the Possible Role of Mesothelium as a Surface for Flowing Blood

J M F Clarke; R M Pittilo; S J Machin; N Woolf

doi:10.1055/s-0038-1661019

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1984; 51(01): 057-060
DOI: 10.1055/s-0038-1661019

Original Article

Schattauer GmbH Stuttgart

A Study of the Possible Role of Mesothelium as a Surface for Flowing Blood

Authors

J M F Clarke

The Departments of Surgical Studies, Histopathology and Haematology, The Middlesex Hospital Medical School, London, U. K.
R M Pittilo

The Departments of Surgical Studies, Histopathology and Haematology, The Middlesex Hospital Medical School, London, U. K.
S J Machin

The Departments of Surgical Studies, Histopathology and Haematology, The Middlesex Hospital Medical School, London, U. K.
N Woolf

The Departments of Surgical Studies, Histopathology and Haematology, The Middlesex Hospital Medical School, London, U. K.

Abstract

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Summary

Mouse mesothelium has been examined as a surface for supporting blood flow. We have examined ten pieces of intact mesothelium and ten pieces of damaged mesothelium following 10 min exposure to flowing blood in a Baumgartner chamber. Scanning electron microscopy of the intact specimens demonstrated no adhering blood platelets, whereas the damaged specimens were found to be covered with large numbers of adhering platelets. In addition we have demonstrated that undamaged mesothelium does not appear to be morphologically altered after exposure to blood, and that undamaged mesothelium produces significantly more prostacyclin than damaged control. These findings suggest that mesothelial cells hold promise as a lining for prosthetic vascular implants.

Key Words

Mesothelium - Prostacyclin - Scanning electron microscopy - Platelets - Artificial circulation - Vascular prosthesis

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References

References
1 Kidson IG. The effect of wall mechanical properties on patency of arterial grafts. Ann R Coll Surg Eng 1983; 65: 24-29
2 Berger K, Sauvage LR, Rao AM, Wood SJ. Healing of arterial prostheses in man; its incompleteness. Ann Surg 1972; 175: 118-127
3 Moncada S, Higgs EA, Vane JR. Human arterial and venous tissues generate prostacyclin (prostaglandin X), a potent inhibitor of platelet aggregation. Lancet 1977; 1: 18-20
4 Lokskutoff DJ, Edgington TS. Synthesis of a fibrinolytic activators and inhibitors by endothelial cells. Proc Natl Acad Sci USA 1977; 74: 3903-3907
5 Kiesel W. Human plasma protein C: isolation, characterisation and mechanism of activation by alpha thrombin. J Clin Invest 1979; 64: 761-769
6 Whitaker D, Papadimitrou JM, Walters MN-I. The mesothelium. Its fibrinolytic properties J Pathol 1982; 136: 291-299
7 Baumgartner HR. The role of blood flow in a platelet adhesion, fibrin deposition and formation of mural thrombus. Microvasc Res 1973; 5: 167-179
8 Defreyn G, Vergara Dauden M, Machin SJ, Vermylen J. A plasma factor in uraemia which stimulates prostacyclin release from cultured endothelial cells. Thromb Res 1980; 19: 695-699
9 Khan RH, Burkel WE. Propagation of pseudointimal linings of vascular prosthetic grafts. In Vitro 1973; 8: 451-458
10 McCollum CN, Kester RC, Rajah SM, Learoyd P, Pepper M. Arterial graft maturation: the duration of thrombotic activity in Dacron aortobifemoral grafts measured by platelet and fibrinogen kinetics. Br J Surg 1981; 68: 61-64
11 Harker LA, Slichter SJ, Sauvage LR. Platelet consumption by arterial prostheses: the effects of endothelialisation and pharmacologic inhibition of platelet function. Ann Surg 1977; 186: 594-601
12 Sauvage LR, Berger K. The presence of endothelium in an axillary femoral knitted Dacron graft with an external velour surface. Ann Surg 1975; 182: 749-753
13 Adachi M, Suzuki M, Kennedy JH. Comparisons of cultured cells derived from autologous tissues of various organs. J Surg Res 1975; 11: 483-491
14 Moore KL. Formation of the trilaminar embryo. In: The Developing Human. Saunders WB. 3. Ed Philadelphia: 1982. pp 53-59
15 Haar JL, Ackerman GA. A phase and electron microscopic study of vasculogenesis and erythropoesis in the yolk sac of the mouse. Anat Rec 1970; 170: 199-223
16 Willis RA. In: The borderland of Embryology and Pathology. Butterworths; London: 1962. pp 54-59
17 Cotran RS, Majno G. Studies on the intercellular junctions of mesothelium and endothelium. Protoplasma 1967; 63: 45-51
18 Simionescu M, Simionescu N. Organisation of cell junctions in the peritoneal mesothelium. J Cell Biology 1977; 74: 98-110
19 Casley-Smith JR. The dimensions and numbers of small vesicles in cells, endothelial and mesothelial and the significance of these for the endothelial permeability. J Microsc 1969; 90: 251-269
20 Schwarz W. The surface film on the mesothelium of the serous membranes of the rat. Z Zellforsch 1974; 147: 595-597
21 Shirahama T, Cohen AS. The role of mucopolysaccharides in vesicle architecture and endothelial transport. J Cell Biol 1972; 52: 198-206
22 Baradi AF, Hope J. Ultrastructure of mesothelium. Exp Cell Res 1964; 34: 33-44
23 Odor DL. Observations of rat mesothelium with electron and phase microscopes. Am J Anat 1954; 95: 433-465
24 Baradi AF, Rao SN. A scanning electron microscope study of mouse peritoneal mesothelium. Tissue Cell 1976; 8: 159-162
25 Madison LD, Bergstrom-Porter B, Torres R, Shelton E. Regulation of the surface topography of mouse peritoneal cells. J Cell Biol 1979; 82: 783-797
26 Fujimoto S, Yamamoto K, Takeshige Y. Electron microscopy of endothelial microvilli of large arteries. Anat Rec 1975; 183: 259-266
27 Pittilo RM, Mackie IJ, Rowles PM, Machin SJ, Woolf N. Effects of cigarette smoking on the ultrastructure of rat thoracic aorta and its ability to produce prostacyclin. Thromb Haemostas 1982; 48: 173-176
28 Weibel EW, Palade GE. New cytoplasmic components in arterial endothelia. J Cell Biol 1964; 23: 101-112
29 Herman AG, Claeys M, Moncada S, Vane JR. Biosynthesis of Prostacyclin (PGI2) and 12 1 Hydroxy. 18: 439-444
30 Coene MC, Solheid C, Claeys M, Herman AG. Prostaglandin production by cultured mesothelial cells. Arch Int Pharmacodyn 1981; 249: 316-318
31 Warren BA. Fibrinolytic properties of vascular endothelium. Br J Exp Pathol 1963; 44: 365-371
32 Raftery AT. Regeneration of peritoneum: a fibrinolytic study. J Anat 1979; 129: 659-664