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Thromb Haemost 1984; 51(01): 110-114
DOI: 10.1055/s-0038-1661034
Original Article
Schattauer GmbH Stuttgart

A Comparative Study Using Immunological and Biological Assays of the Haemostatic Responses to DDAVP Infusion Venous Occlusion and Exercise in Normal Men

C V Prowse
The Edinburgh and South-East Scotland Blood Transfusion Service Edinburgh, Great Britain
,
A Farrugia
The Edinburgh and South-East Scotland Blood Transfusion Service Edinburgh, Great Britain
,
F E Boulton
The Edinburgh and South-East Scotland Blood Transfusion Service Edinburgh, Great Britain
,
J Tucker
*   The Department of Haematology, Royal Infirmary, Edinburgh, Great Britain
,
C A Ludlam
*   The Department of Haematology, Royal Infirmary, Edinburgh, Great Britain
,
M McLaren
**   The Department of Medicine, Royal Infirmary, Glasgow, Great Britain
,
J J F Belch
**   The Department of Medicine, Royal Infirmary, Glasgow, Great Britain
,
C R M Prentice
**   The Department of Medicine, Royal Infirmary, Glasgow, Great Britain
,
J Dawes
***   The MRC/SNBTS Blood Components Assay Group, Forrest Road, Edinburgh, Great Britain
,
I R MacGregor
****   The Scottish National Blood Transfusion Service, Headquarters Laboratory, Forrest Road, Edinburgh, Great Britain
› Author Affiliations
Further Information

Publication History

Received 20 June 1983

Accepted 09 December 1983

Publication Date:
26 July 2018 (online)

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Summary

In a group of six normal male volunteers, infusion of DDAVP, venous occlusion and exercise were shown to increase plasma levels of factor VIII and plasminogen activator, activity and antigen, to different extents and at differing rates. Any mechanisms suggested to explain release of these proteins by various stimuli should account for such differences.

All three stimuli could also increase plasma levels of prostacyclin metabolites, although this was only significant for high doses of DDAVP. Other potential endothelial markers, such as fibronectin and thrombospondin, showed no specific increase after any of the stimuli.

Key Words

Plasminogen activator - Factor VIII - DDAVP - Venous occlusion - Exercise
 

  • References

  • 1 Cash JD, Gader AM A, Da Costa J. The Release of Plasminogen Activator and Factor VIII by LVP, AVP, DDAVP, ATIII and OT in Man. Br J Haematol 1974; 27: 363-364
    PubMedGoogle Scholar
  • 2 Mannucci PM, Aberg M, Nilsson IM, Robertson B. Mechanism of Plasminogen Activator and Factor VIII Increase After Vasoactive Drugs. Br J Haematol 1975; 30: 81-93
    CrossrefPubMedGoogle Scholar
  • 3 Nilsson IM, Vilhardt H, Holmberg L, Astedt B. Association between Factor VIII Related Antigen and Plasminogen Activator. Acta Med Scand 1982; 211: 105-112
    PubMedGoogle Scholar
  • 4 Prowse CV, Sas G, Gader AM A, Cort JH, Cash JD. Specificity in the Factor VIII Response to Vasopressin Infusion in Man. Br J Haematol 1979; 41: 437-447
    CrossrefPubMedGoogle Scholar
  • 5 Cash JD, Gader AM A, Mulder JL, Cort JH. Structure-Activity Relations of the Fibrinolytic Response to Vasopressin in Man. Clin Sei Mol Med 1978; 54: 403-409
    PubMedGoogle Scholar
  • 6 Brozovic M. Physiological Mechanisms in Coagulation and Fibrinolysis. Br Med Bull 1977; 33: 231-238
    CrossrefPubMedGoogle Scholar
  • 7 Mannucci PM, Ruggeri ZM, Pareti FI, Capitanio A. DDAVP: A New Pharmacological Approach to the Management of Haemophilia and von Willebrand’s Disease. Lancet 1977; 1: 689-872
    PubMedGoogle Scholar
  • 8 Kobayashi I. Treatment of Haemophilia A and von Willebrand’s Disease Patients with an Intranasal Dripping of DDAVP. Thromb Res 1979; 16: 775-780
    CrossrefPubMedGoogle Scholar
  • 9 Brommer EJ P, Barrett-Bergshoeff MM, Allen RA, Schicht I, Bertina RM, Schalekamp MA D H. The Use of Desmopressin Acetate as a Test of Fibrinolytic Capacity of Patients - Analysis of Responders and Non-Responders. Thromb Haemostas 1982; 48: 156-161
    PubMedGoogle Scholar
  • 10 Rijken DC, Wijngaards G, Welbergen J. Relationship between Tissue Plasminogen Activator and the Activators in Blood and Vascular Wall. Thromb Res 1980; 18: 815-830
    CrossrefPubMedGoogle Scholar
  • 11 Kluft C. Studies on the Fibrinolytic System in Human Plasma: Quantitative Determination of Plasminogen Activators and Proactivators. Thromb Haemostas 1979; 41: 365-383
    PubMedGoogle Scholar
  • 12 MacGregor I, Pepper D. A Direct Functional Assay and an Immunoassay for Plasminogen Activators. Haemostasis 1982; 11 suppl (Suppl. 01) Abstract 117
    PubMedGoogle Scholar
  • 13 Peake IR. In: The Haemophilias. Bloom A. (Ed) Churchill Livingstone; 1982. pp 92-105
  • 14 Belch JJ F, Small M, McKenzie F, Hill PA, Lowe GD O, McIntyre DE, Forbes CD, Prentice CR M. DDAVP Stimulates Prostacyclin Release. Thromb Haemostas 1982; 47: 122-123
    PubMedGoogle Scholar
  • 15 Greaves M, Preston F. Plasma 6-Keto-Prostaglandin F1: Fact or Fiction. Thromb Res 1982; 26: 145-157
    CrossrefPubMedGoogle Scholar
  • 16 Prowse CV, Douglas JG, Forrest JA H, Forsling ML. Haemostatic Effects of Lysine Vasopressin and Triglycyl Lysine Vasopressin Infusion in Patients with Cirrhosis. Eur J Clin Invest 1980; 10: 49-54
    CrossrefPubMedGoogle Scholar
  • 17 Macfarlane DE, Stibbe J, Kirby EP, Zucker MB, Grant RA, McPherson J. A Method for Assaying von Willebrand Factor (ristocetin cofactor). Thrombos Diathes Haemorrh 1975; 34: 306-308
    PubMedGoogle Scholar
  • 18 Rotblat F, Tuddenham EG. Immunological Studies of Factor VIII Coagulant Activity (VIII:C): 1: Assays Based on a Haemophilic and an Acquired Antibody to VIII:C. Thromb Res 1981; 21: 431-445
    CrossrefPubMedGoogle Scholar
  • 19 Laurell CB. Electroimmunoassay. Scand J Clin Lab Invest 1972; 29 suppl (Suppl. 124) 21-37
    CrossrefPubMedGoogle Scholar
  • 20 Clauss A. Gerinnungsphysiologische Schnellmethode zur Bestimmung des Fibrinogens. Acta Haematol 1957; 17: 237-246
    CrossrefPubMedGoogle Scholar
  • 21 Gomall AG, Bardawill CJ, David MM. Determination of Serum Proteins by Means of the Biuret Reaction. J Biol Chem 1949; 177: 751-766
    PubMedGoogle Scholar
  • 22 MacGregor IR, Prowse CV. Tissue Plasminogen Activator in Human Plasma Measured by Radioimmunoassay. Thromb Res 1983; 31: 461-474
    CrossrefPubMedGoogle Scholar
  • 23 Prowse CV, Pepper DS, Dawes J. Prevention of the Platelet AlphaGranule Release Reaction by Membrane Active Drugs. Thromb Res 1982; 25: 219-227
    CrossrefPubMedGoogle Scholar
  • 24 Dawes J, Clemetson KJ, Gogstad GO, McGregor J, Clezardin O, Prowse CV, Pepper DS. A Radioimmunoassay for Thrombospondin Used in a Comparative Study of Thrombospondin, B-Thromboglobu- lin and Platelet Factor 4 in Healthy Volunteers. Thromb Res 1983; 29: 569-581
    CrossrefPubMedGoogle Scholar
  • 25 Robertson BA, Pandolfi M, Nilsson IM. Fibrinolytic Capacity in Healthy Volunteers as Estimated from the Effect of Venous Occlusion of Arms. Acta Chir Scand 1972; 138: 429-440
    PubMedGoogle Scholar
  • 26 Rijken DC, Collen D. Purification and Characterisation of the Plasminogen Activator Secreted by Human Melanoma Cells in Culture. J Biol Chem 1981; 256: 7035-7041
    PubMedGoogle Scholar
  • 27 Rijken DC, Juhan-Vague I, De Cock F, Collen D. Measurement of Human Tissue-Type Extrinsic Plasminogen Activator by a Two-Site Immunoradiometric Assay. J Lab Clin Med 1983; 101: 274-284
    PubMedGoogle Scholar
  • 28 Marsh N, Gaffney P. Some Observations on the Release of Extrinsic and Intrinsic Plasminogen Activators During Exercise in Man. Haemostasis 1980; 9: 238-247
    PubMedGoogle Scholar
  • 29 Holmberg L, Nilsson IM, Astedt B. Immunoreactive Tissue Activator and Urokinase in Plasma after Various Stimuli. Haemostasis 1982; 11 suppl (Suppl. 01) abstr 55
    PubMedGoogle Scholar
  • 30 Davis GL. Haemostatic and Physiological Changes Induced by Various Lengths of Venous Occlusion (abstr.). Fed Proc 1982; 41: 704
    PubMedGoogle Scholar
  • 31 Ruggeri ZM, Mannucci PM, Lombardi R, Federici AB, Zimmerman TS. Multimeric Composition of Factor VIII - von Willebrand Factor Following Administration of DDAVP - Implications for Patho physiology and Therapy of von Willebrand’s Disease Subtype. Blood 1982; 59: 1272-1278
    PubMedGoogle Scholar
  • 32 Todd AS. The Histological Localisation of Fibrinolysin Activator. J Pathol 1959; 281-283
    PubMedGoogle Scholar
  • 33 Jaffe EA, Hoyer LW, Nachman RL. Synthesis of Antihaemophilic Factor Antigen by Cultured Human Endothelial Cells. J Clin Invest 1973; 52: 2757-2764
    CrossrefPubMedGoogle Scholar
  • 34 Jaffe EA, Mosher DF. Synthesis of Fibronectin by Cultured Human Endothelial Cells. J Exp Med 1978; 147: 1779-1791
    CrossrefPubMedGoogle Scholar
  • 35 Mosher DF, Doyle MJ, Jaffe EA. Synthesis and Secretion of Thrombospondin by Cultured Human Endothelial Cells. J Cell Biol 1982; 93: 343-348
    CrossrefPubMedGoogle Scholar
  • 36 Chan TK, Chan V. Antithrombin III, The Major Modulator of Intravascular Coagulation, is Synthesised by Human Endothelial Cells. Thromb Haemostas 1981; 46: 504-506
    PubMedGoogle Scholar
  • 37 Busch C, Dawes J, Pepper DS, Wasteson A. Binding of Platelet Factor 4 to Cultured Human Endothelial Cells. Thromb Res 1980; 19: 129-137
    CrossrefPubMedGoogle Scholar
  • 38 D’Angelo A, Capitanio A, Smith JB, Valsecchi C, Mannucci PM. Effects of Des-Amino-D-Arginine Vasopressin (DDAVP) on Plasma Levels of Platelet and Endothelial Cell Release Products. Thromb Haemostas 1983; 49: 64
    PubMedGoogle Scholar
  • 39 Belch JJ F, McLaren M, Lowe GD O, McIntyre DE, Forbes CD, Prentice CR M. Effect of Des-Amino-D-Arginine Vasopressin (DDAVP) on Plasma Levels of Platelet and Endothelial Cell Release Products - Reply. Thromb Haemostas 1983; 49: 65
    PubMedGoogle Scholar
  • 40 Barrow SE, Heavey DJ, Jacqz E, Blair IA, Dollery CT. Des-Amino-D-Arginine Vasopressin does not Increase Circulating Levels of Prostacyclin or Thromboxane A2 during Infusion in Man. Thromb Haemostas 1983; 50: 622
    PubMedGoogle Scholar
  • 41 Viinka L, Ylikorkala O. Different Results of Plasma 6-Keto Prostaglandin F1Measurements Utilising Two Antisera with Apparently Similar Specificity. Prost Leuk Med 1982; 9: 1-7
    CrossrefPubMedGoogle Scholar
  • 42 Rosenkranz B, Fisher C, Frohlich JC. Prostacyclin Metabolites in Human Plasma. Clin Pharm Thera 1981; 29: 420
    PubMedGoogle Scholar
  • 43 Prowse CV, Dow RC, Sheward WJ, Fink G, Boulton FE. The Plasminogen Activator Response to Drug Infusion and to Direct Hypothalamic Stimulation in Dogs and Rats. Progress in Fibrinolysis. Davidson JF. (Ed) Churchill Livingstone; 1983. 6 104-106
    Google Scholar
  • 44 Ponari O, Civardi E, Megha A, Pini M, Poti R, Dettori AG. Effect of Alpha and Beta-Blocking Drugs on the Clotting and Fibrinolytic Response to Venous Stasis in Man. Br J Haematol 1973; 24: 463-470
    CrossrefPubMedGoogle Scholar
  • 45 Cohen RJ, Epstein SE, Cohen LS, Dennis LH. Alterations of Fibrinolysis and Blood Coagulation Induced by Exercise and the Role of Beta-Adrenergic Receptor Stimulation. Lancet 1968; 2: 1264-1266
    PubMedGoogle Scholar