A Quick Method for Screening Platelet Dysfunctions Using the Whole Blood Lumi-Aggregometer

Carol M Ingerman-Wojenski; Melvin J Silver

doi:10.1055/s-0038-1661048

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1984; 51(02): 154-156
DOI: 10.1055/s-0038-1661048

Original Article

Schattauer GmbH Stuttgart

A Quick Method for Screening Platelet Dysfunctions Using the Whole Blood Lumi-Aggregometer

Carol M Ingerman-Wojenski

The Cardeza Foundation for Haematologic Research, Department of Medicine and Department of Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania, U.S.A.

,

Melvin J Silver

The Cardeza Foundation for Haematologic Research, Department of Medicine and Department of Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania, U.S.A.

› Author Affiliations

Abstract

PDF Download

Summary

Patients who present with a clinical history suggesting a bleeding disorder are often tested initially for a clotting defect rather than for platelet dysfunction, due to the length of time necessary to complete a platelet function study in platelet-rich plasma. We have developed a sensitive method for measuring platelet aggregation and release of ATP employing the Whole Blood Lumi-Aggregometer. This method makes it possible to quickly detect patients who require further study for possible platelet function disorders such as cyclooxygenase deficiency, storage-pool defect, thrombasthenia, and von Willebrand’s disease. The results obtained with this electrical impedance instrument do not differ from those obtained with the conventional optical method. However, it is now possible to recognize a platelet function defect within 30 min of obtaining a 5 ml sample of citrated whole blood. Further, platelets of unusual size or density are not lost to testing through centrifugation.

Keywords

Platelet aggregation - Release reaction - Electrical impedance method - Platelet dysfunction

PDF (162 kb)

References

References
1 Born GV R. Quantitative investigation into the aggregation of blood platelets. J Physiol 1962; 16: 68
2 O’Brien JR. Platelet Aggregation. Part II. Some results from a new study J Clin Pathol 1962; 15: 452-455
3 Cardinal DC, Flower RJ. The electronic aggregometer: a novel device for assessing platelet behavior in blood. J Pharmacol Methods 1980; 3: 135-158
4 Komecki E, Niewiarowski S, Morinelli TA, Kloczewiak M. Effects of chymotrypsin and adenosine diphosphate on the exposure of fibrinogen receptors on normal human and Glanzmann’s thrombasthenia platelets. J Biol Chem 1981; 256: 5696-5701
5 Ingerman CM, Smith JB, Shapiro S, Sedar A, Silver MJ. Hereditary abnormality of platelet aggregation attributable to nucleotide storage pool deficiency. Blood 1978; 52: 332-344
6 Feinman RD, Lubowsky J, Caro IF, Zabinski MP. The lumiaggregometer: a new instrument for simultaneous measurement of secretion and aggregation. J Lab Clin Med 1977; 90: 125-129
7 Ingerman-Wojenski C, Smith JB, Silver MJ. Evaluation of electrical aggregometry: comparison with optical aggregometry, secretion of ATP, and accumulation of radiolabeled platelets. J Lab Clin Med 1983; 101: 44-52
8 Ingerman CM, Smith JB, Silver MJ. Direct measurement of platelet secretion in whole blood. Thromb Res 1979; 16: 335-344
9 Ingerman-Wojenski CM, Smith JB, Silver MJ. Difficulty in detecting inhibition of platelet aggregation by the impedance method. Thromb Res 1982; 28: 426-432