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Thromb Haemost 1984; 51(02): 266-268
DOI: 10.1055/s-0038-1661073
Original Article
Schattauer GmbH Stuttgart

Histidine-Rich Glycoprotein Modulates the Anticoagulant Activity of Heparin in Human Plasma

H R Lijnen
The Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, Belgium
,
B Van Hoef
The Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, Belgium
,
D Collen
The Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, Belgium
› Author Affiliations
Further Information

Publication History

Received 09 January 1984

Accepted 22 February 1984

Publication Date:
19 July 2018 (online)

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Summary

Addition of 0.05 IU of heparin per ml normal plasma prolongs its thrombin time from 20 to 27s, but that of plasma specifically depleted in histidine-rich glycoprotein (by immunoadsorption) from 20 to 180s. Reconstitution of the depleted plasma with normal plasma or with purified histidine-rich glycoprotein normalizes its thrombin time.

In 54 plasma samples from hospitalized patients a significant negative correlation was found between the anticoagulant activity of heparin measured by thrombin inhibition and the plasma level of histidine-rich glycoprotein (r = 0.69). It is concluded that the level of histidine-rich glycoprotein modulates the anticoagulant activity of heparin in human plasma to an extent which appears to be pharmacologically and possibly clinically relevant.

Keywords

Histidine-rich glycoprotein - Heparin - Anticoagulant activity
 

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