Studies on the Procurement of Coagulation Factor VIII: Selective Precipitation of Factor VIII with Hydrophilic Polymers

A Farrugia; B Griffin; D Pepper; C Prowse

doi:10.1055/s-0038-1661096

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1984; 51(03): 338-342
DOI: 10.1055/s-0038-1661096

Original Article

Schattauer GmbH Stuttgart

Studies on the Procurement of Coagulation Factor VIII: Selective Precipitation of Factor VIII with Hydrophilic Polymers

A Farrugia

^*The Edinburgh & S-E Scotland Blood Transfusion Service, Royal Infirmary, Edinburgh, Scotland

,

B Griffin

^**Scottish National Blood Transfusion Service Headquarters Unit Laboratory, Edinburgh, Scotland

,

D Pepper

^**Scottish National Blood Transfusion Service Headquarters Unit Laboratory, Edinburgh, Scotland

,

C Prowse

^*The Edinburgh & S-E Scotland Blood Transfusion Service, Royal Infirmary, Edinburgh, Scotland

› Author Affiliations

Abstract

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Summary

Early work on the purification of factor VIII using polyethylene glycol (PEG) indicated that other polymers might also be used to precipitate factor VIII leaving fibrinogen in solution. Recently polyvinylpyrrolidone (PVP) has also been advocated for this purpose.

In this study, different concentrations and molecular weights of hydroxyethyl starch, dextran, PEG, PVP, Ficoll, Percoll and albumin were examined for their ability to precipitate the factor VIII complex from cooled (not frozen) fresh CPD plasma. At optimal concentrations near quantitative recovery of VIIIR:Ag and VIII: CAg was achieved in 2 hr with minimal precipitation of fibrinogen or total protein. The best separations were observed with hydroxyethyl starch, albumin or Ficoll. PVP and PEG gave inferior purifications. Results for cryoprecipitate and intermediate-purity factor VIII concentrate were inferior to those obtained with plasma. Simple pre-concentration of plasma prior to chilling is an attractive alternative for large scale continuous production.

Keywords

Factor VIII - Fibrinogen - Polymer - Protein precipitation

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References

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