Morphometric Analysis of Platelets in Bernard-Soulier Syndrome: Size and Configuration in Patients and Carriers

M McGill; G A Jamieson; J Drouin; M S Cho; G A Rock

doi:10.1055/s-0038-1661132

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1984; 52(01): 037-041
DOI: 10.1055/s-0038-1661132

Original Article

Schattauer GmbH Stuttgart

Morphometric Analysis of Platelets in Bernard-Soulier Syndrome: Size and Configuration in Patients and Carriers

Authors

M McGill

¹The American Red Cross Blood Services Laboratories, Bethesda, MD, U. S. A.

²The University of Cincinnati Medical Center, the Paul I. Hoxworth Blood Center, Cincinnati, Ohio, U.S.A.
G A Jamieson

¹The American Red Cross Blood Services Laboratories, Bethesda, MD, U. S. A.
J Drouin

⁴The Ottawa Centre, Canadian Red Cross Blood Transfusion Services, Ottawa, Canada
M S Cho

¹The American Red Cross Blood Services Laboratories, Bethesda, MD, U. S. A.
G A Rock

³The Ottawa General Hospital, Ottawa, Canada

⁴The Ottawa Centre, Canadian Red Cross Blood Transfusion Services, Ottawa, Canada

Abstract

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Summary

Quantitative ultrastructural morphometric analysis has been carried out on thin sections of platelets from two Bernard-Soulier sisters and their parents. Measurements were made for the major and minor axes, axial ratios, cross-sectional circumference and cross-sectional area. Platelets were collected either into CPD anticoagulant or directly into glutaraldehyde. The results confirm that Bemard-Soulier platelets are significantly larger than normals (p <0.05) in all the parameters mentioned and indicate (I) that the morphology of platelets from Bernard-Soulier patients is affected by the presence of anticoagulant, and (II) a more spherocytic configuration is characteristic of Bemard-Soulier disease both in patients and carriers.

Keywords

Bemard-Soulier - Ultrastructure - Morphometric analysis - Platelets

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References

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