Download PDF
Thromb Haemost 1984; 52(03): 232-235
DOI: 10.1055/s-0038-1661185
Original Article
Schattauer GmbH Stuttgart

Influence of Some Plasma Fatty Acids on the Phospholipid Fatty Acid Pattern of Human Platelets – An “Ex Vivo” Experience

Juana Vallés
1   The Research Center, Ciudad Sanitaria “La Fe”, Valencia, Spain
,
Justo Aznar
2   Department of Clinical Pathology, Ciudad Sanitaria “La Fe”, Valencia, Spain
,
M Teresa Santos
1   The Research Center, Ciudad Sanitaria “La Fe”, Valencia, Spain
› Author Affiliations
Further Information

Publication History

Received 17 May 1984

Accepted 02 August 1984

Publication Date:
19 July 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

Some correlations between plasma and platelet fatty acids (FA) were evaluated “ex vivo” in 94 normal subjects.

The highest relationships between total FA from plasma and platelets were found for 18:1 (r = 0.74) and 18:2 (r = 0.67). Low correlations were obtained for free fatty acids (FFA). The most significant correlations between fatty acids esterifying plasma and platelet phospholipids were found for the 18:1 (r = 0.66); 18:2 (r = 0.74) and 20:5 (r = 0.66).

Our results suggest that the platelet phospholipid FA could be more easily modified by plasma variation in the FA composition of phospholipids than by variations in the plasma FA composition of the FFA fraction. In addition, the incorporation of FA from plasma into the platelet phospholipids “in vivo” may take place through an acylation-deacylation process and also by the incorporation of whole plasma phospholipid molecules into the platelets, probably through an exchange of plasma lipoproteins and platelets. Finally, arachidonic acid seems to have a different and selective way of incorporation into platelet phospholipids.

Key words

Platelets - Fatty acids - Phospholipids - Plasma fatty acids
 

  • References

  • 1 Schick BP, Schick PK. Cholesterol and phospholipid biosynthesis in guinea pig megakaryocytes. Biochim Biophys Acta 1981; 663: 249-254
    CrossrefPubMedGoogle Scholar
  • 2 Deykin D, Desser RK. The incorporation of acetate and palmitate into lipids by human platelets. J Clin Invest 1968; 47: 1590-1602
    CrossrefPubMedGoogle Scholar
  • 3 Majerus PW, Smith MB, Clamon GH. Lipid metabolism in human platelets I. Evidence for a complete fatty acid synthesizing system. J Clin Invest 1969; 48: 156-164
    CrossrefPubMedGoogle Scholar
  • 4 Lewis N, Majerus PW. Lipid metabolism in human platelets. II De novo phospholipid synthesis and effect of thrombin on the patterns of synthesis. J Clin Invest 1969; 48: 2114-2123
    CrossrefPubMedGoogle Scholar
  • 5 Nordoy A. Plasma and platelet lipids in man. Haemostasis 1973; 2: 103-117
    PubMedGoogle Scholar
  • 6 Shattil SJ, Anaya-Galindo R, Bennett J, Colman RW, Cooper RA. Platelet hypersensitivity induced by cholesterol incorporation. J Clin Invest 1975; 55: 636-643
    CrossrefPubMedGoogle Scholar
  • 7 Bereziat G, Chambaz J, Trugnon G, Pepin D. Polonvski. Turnover of phospholipid linoleic and arachidonic acids in human platelets from plasma lecithins. J Lipid Res 1978; 19: 495-500
    PubMedGoogle Scholar
  • 8 Bills TK, Smith JB, Silver M. Metabolism of [14c] arachidonic acid by human platelets. Biochim Biophys Acta 1976; 424: 303-314
    CrossrefPubMedGoogle Scholar
  • 9 Bills TK, Smith JB, Silver MJ. Platelet uptake, release and oxidation of [14c] arachidonic acid. In: Prostaglandins in Haematology. Silver MJ, Smith JB, Kocsis JJ. (Eds) Spectrum Publications; New York: 1977. pp 27-55
    Google Scholar
  • 10 Derkensen A, Cohen P. Extensive incorporation of (2-14C) mevalonic acid into cholesterol precursors by human platelets in vitro. J Biol Chem 1973; 248: 7396-7403
    PubMedGoogle Scholar
  • 11 Grøttum KA, Nordoy A, Hellem A. Lipid infusions in man. Blood platelet uptake-of lipid and effects on platelet functions Thrombos Diathes Haemorrh 1973; 29: 701-711
    PubMedGoogle Scholar
  • 12 Nordoy A, Strom E, Gjesdal K. The effect of alimentary hyperlipaemia and primary hypertriglyceridaemia on platelets in man. Scand J Haematol 1974; 12: 329-340
    PubMedGoogle Scholar
  • 13 McKean ML, Smith JB, Silver MJ. Phospholipid biosynthesis in human platelets. J Biol Chem 1982; 257: 11278-11283
    PubMedGoogle Scholar
  • 14 Elsbach P, Pettis P, Marcus A. Lysolecithin metabolism by human platelets. Blood 1971; 37: 675-683
    PubMedGoogle Scholar
  • 15 Valles J, Aznar J, Santos MT. Effect of aspirin on platelet phospholipids. Thromb Haemostas 1976; 36: 628-633
    PubMedGoogle Scholar
  • 16 Aznar J, Valles J, Santos MT. The effect of prolonged and uniform diets on the lipid composition of platelets. Biometrica 1978; 3: 121-130
    PubMedGoogle Scholar
  • 17 Morrison WR, Smith LM. Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. J Lipid Res 1964; 5: 600-608
    PubMedGoogle Scholar
  • 18 Aznar J, Valles J, Santos MT, Fernandez MA. Abnormal platelet lipid pattern in chronic heart disease patients. Thromb Res 1980; 505-512
    PubMedGoogle Scholar
  • 19 Nordoy A, Bjorge JM, Strom E. Comparison of the main lipids in platelets and plasma in man. Acta Med Scand 1973; 193: 59-64
    PubMedGoogle Scholar
  • 20 Spector AA, Hoak JC, Warner ED, Fry GL. Utilization of long-chain free fatty acids by human platelets. J Clin Invest 1970; 49: 1489-1496
    CrossrefPubMedGoogle Scholar
  • 21 Cohen P, Derksen A, Van Den Bosch. Pathway of fatty acid metabolism in human platelets. J Clin Invest 1970; 49: 128-139
    CrossrefPubMedGoogle Scholar
  • 22 Needleman SW, Spector AA, Hoak JC. Enrichment of human platelets phospholipids with linoleic acid diminishes thromboxane release. Prostaglandins 1982; 24: 607-622
    CrossrefPubMedGoogle Scholar
  • 23 Seyberth HW, Oelz O, Kennedy T, Sweetman BJ, Danon A, Frolich C, Heimberg M, Oates JA. Increased arachidonate in lipids after administration to man: effects on prostaglandin biosynthesis. Clin Pharmacol Ther 1975; 18: 521-529
    CrossrefPubMedGoogle Scholar