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Thromb Haemost 1984; 52(03): 243-249
DOI: 10.1055/s-0038-1661188
Original Article
Schattauer GmbH Stuttgart

Plasminogen Activator and Plasminogen Activator Inhibitor Associated with Granulomatous Inflammation: A Study with Murine Leprosy

S Izaki
The Department of Dermatology, Iwate Medical University School of Medicine, Morloka, Iwate, Japan
,
T Hibino
The Department of Dermatology, Iwate Medical University School of Medicine, Morloka, Iwate, Japan
,
Y Isozaki
The Department of Dermatology, Iwate Medical University School of Medicine, Morloka, Iwate, Japan
,
P S Hsu
The Department of Dermatology, Iwate Medical University School of Medicine, Morloka, Iwate, Japan
,
M Izaki
The Department of Dermatology, Iwate Medical University School of Medicine, Morloka, Iwate, Japan
,
O Matsuo
*   The Department of Physiology, Kinki University School of Medicine, Sayama, Osaka, Japan
› Author Affiliations
Further Information

Publication History

Received 02 December 1983

Accepted 08 August 1984

Publication Date:
19 July 2018 (online)

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Summary

Plasminogen activator that is associated with the development of hypersensitivity granulomas (gPA) was partially purified from a saline soluble fraction of murine lepromas elicited in “resistant” mice, C57BL/6N. The gPA was shown to consist of two subspecies (23,000 and 48,000 in molecular weight) with essentially identical enzymologic properties. The gPA was found to be a relatively heat stable weakly alkaline serine proteinase with trypsin-like characteristics in the specificity for synthetic substrates and proteinase inhibitors. It showed a high affinity for H- D-Ile-Pro-Arg-pNA (Km = 1.4 × 10-4 M) H-D-Val-Leu-Lys- pNA (Km = 5.2 × 10-4 M), and L-pyroGlu-Gly-Arg-pNA (Km = 9.3 × 10-4 M). The gPA did not demonstrate antigenic cross reaction with urokinase-type or tissue-type plasminogen activator.

Two distinct enzymatic regulators of the gPA were also demonstrated in the saline soluble fraction of the hypersensitivity granulomas. The gPA and its regulation are assumed to be correlated with macrophage activation in the hypersensitivity granulomas

Key words

Plasminogen activator - Plasminogen activator inhibitor - Inflammation - Hypersensitivity granulomas - Murine leprosy
 

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