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Thromb Haemost 1984; 52(03): 350-353
DOI: 10.1055/s-0038-1661212
Original Article
Schattauer GmbH Stuttgart

Improved Assay Conditions for Automated Antithrombin III Determinations with the Chromogenic Substrate S-2238

H van Voorthuizen
1   The KabiVitrum B. V. Diagnostica, Amsterdam
,
C Kluft
2   The Gaubius Institute T. N. O., Leiden, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 02 July 1984

Accepted 18 October 1984

Publication Date:
19 July 2018 (online)

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Summary

Assays for antithrombin III activity using the chromogenic substrate S-2238 were automated in various modifications on various instruments. Standard curves for the assay showed a deviating “0% point” in several but not all modifications. Addition of polyethylene glycol 6000/8000 (0.40-1.0%) minimizes this deviation. However, the deviation of the 0% point was not completely abolished in every modification of the test. Examination of Lineweaver-Burk plots showed nonlinearity at high concentrations of S-2238 as used in the antithrombin III assays. Addition of Tween-80 (0.01%) restores the Lineweaver-Burk plot and, at the same time, the standard curve in the assays is found to be linear. Therefore, we recommend the use of PEG 6000/8000 as well as Tween-80 in automated assay systems.

Key words

Antithrombin III - Thrombin - Chromogenic substrates
 

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