ADP Induced Depolarization of Human Platelet Membrane Potential

June D Wencel-Drake; Harold Feinberg

doi:10.1055/s-0038-1661240

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1985; 53(01): 075-079
DOI: 10.1055/s-0038-1661240

Original Article

Schattauer GmbH Stuttgart

ADP Induced Depolarization of Human Platelet Membrane Potential

June D Wencel-Drake

The Department of Pharmacology, University of Illinois College of Medicine, Chicago, Illinois,

,

Harold Feinberg

The Department of Pharmacology, University of Illinois College of Medicine, Chicago, Illinois,

› Author Affiliations

Abstract

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Summary

The transmembrane potential of human blood platelets suspended in plasma was investigated by studying the distribution of a radiolabeled permeant ion [14C] thiocyanate. The membrane potential of resting platelets was found to be -54.50 mV ± 9.23 S.D. with a range of -39 to -76 mV (n = 27). The possibility that platelet activation alters membrane potential or that changes in membrane potential serve as an activation trigger was investigated. Stimulation by ADP (10 ¼M) resulted in a significant (p :0.05) depolarization of the membrane potential. Preincubation with 6 mM EGTA failed to inhibit ADP-induced depolarization even though EGTA effectively prevented primary and secondary aggregation but not shape change. Preincubation with PGE₁ inhibited shape change, aggregation, and the ADP- induced depolarization. No significant change in membrane potential was observed following stimulation by epinephrine (50 ¼M). These results suggest that the initial interaction of ADP and its receptor may involve an inward positive current which can be determined by thiocyanate distribution.

Key words

Human blood platelets - Transmembrane potential - Platelet activation - Thiocyanate

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References

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