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Thromb Haemost 1985; 53(01): 122-125
DOI: 10.1055/s-0038-1661250
Original Article
Schattauer GmbH Stuttgart

Purification of a Specific Placental Plasminogen Activator Inhibitor by Monoclonal Antibody and Its Complex Formation with Plasminogen Activator

B Åstedt
*   The Research Laboratories of the Department of Gynecology and Obstetrics, University Hospital of Lund, Lund, Sweden
,
Ingegerd Lecander
*   The Research Laboratories of the Department of Gynecology and Obstetrics, University Hospital of Lund, Lund, Sweden
,
T Brodin
**   The Department of Clinical Chemistry, University Hospital of Lund, Lund, Sweden
,
A Lundblad
**   The Department of Clinical Chemistry, University Hospital of Lund, Lund, Sweden
,
Karin Löw
**   The Department of Clinical Chemistry, University Hospital of Lund, Lund, Sweden
› Author Affiliations
Further Information

Publication History

Received 23 July 1984

Accepted 03 December 1984

Publication Date:
18 July 2018 (online)

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Summary

A monoclonal antibody of IgG2a-type was obtained against a specific fast acting plasminogen activator inhibitor found in placenta. The placental inhibitor was purified by affinity chromatography using the monoclonal antibody and additionally in a FPLC-system. A strong complex formation was found between the inhibitor and urokinase and also with the two-chain form of plasminogen activator of the tissue-type. A weaker complex was found between the placental inhibitor and the one- chain form of the tissue-type activator.

Key words

Monoclonal antibody - Plasminogen activator - Coagulation tests - Urokinase - Placental inhibitor
 

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