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DOI: 10.1055/s-0038-1661290
Incorporation of Some Eicosaenoic Acids into Endothelial Cells – Effect on Platelet Inhibitory Activity and Prostacyclin Production
Publication History
Received 09 November 1984
Accepted 05 February 1985
Publication Date:
18 July 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/198502/lookinside/thumbnails/10-1055-s-0038-1661290-1.jpg)
Summary
Primary cultures of endothelial cells from human umbilical veins were grown until confluency. Then, dihomogammalinolenic acid (DHLA or 20:3n-6) and eicosapentaenoic acid (EPA or 20:5n-3), precursors of monoenoic and trienoic prostanoids, respectively, as well as 5,8,11-eicosatrienoic acid (20:3n-9), and isomer of DHLA, were incorporated into endothelial lipids. DHLA-rich endothelial cells had a decreased capacity of prostacyclin production. By contrast EPA- or 20:3n-9-rich endothelial cells were comparable to controls in this respect. DHLA and EPA were efficiently acylated into cell phospholipids and triglycerides at the opposite of 20:3n-9. It is suggested that both DHLA and EPA could alter the liberation of endogenous arachidonic acid for prostacyclin synthesis but this might be counterbalanced in EPA-rich endothelial cells by PGI3 production. We conclude that DHLA enrichment of endothelial cell lipids may impair the possible beneficial effect of the acid upon platelet functions whereas that of EPA would not be modified.
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References
- 1 Moncada S, Herman AG, Higgs EA, Vane JR. Differential formation of prostacyclin (PGX or PGI2) by layers of the arterial wall. An explanation for the anti-thrombotic properties of vascular endothelium Thrombos Res 1977; 11: 323-344
- 2 Moncada S, Vane JR. The role of PGI2 in vascular tissue. Fed Proc 1979; 38: 66-71
- 3 Moncada S, Gryglewski R, Bunting S, Vane JR. An enzyme isolated form arteries transformed prostaglandin endoperoxides to an unstable substance that inhibits platelet aggregation. Nature 1976; 263: 663-665
- 4 Salmon J, Smith D, Flower R, Moncada S, Vane JR. Further studies on the enzymatic conversion of prostaglandin endoperoxides into prostacyclin by porcine aorta microsomes. Biochim Biophys Acta 1978; 523: 250-262
- 5 Willis AL. Nutritional and pharmacological factors in eicosanoid biology. Nutr Rev 1981; 39: 289-301
- 6 Goodnight SH, Harris WS, Connor WE, Illingworth DR. Polyunsaturated fatty acids, hyperlipidemia, and thrombosis. Arteriosclerosis 1982; 2: 87-113
- 7 Silver MJ, Smith JB, Ingerman CU, Kocsis JJ. Arachidonic acid induced human platelet aggregation and prostaglandin formation. Prostaglandins 1973; 4: 863-875
- 8 Lagarde M, Guichardant M, Dechavanne M. Human platelet PGE1 and dihomogammalinolenic acid Comparison to PGE2 and arachidonic acid. Progr Lipid Res 1981; 20: 439-443
- 9 Renaud S, Dumont E, Godsey F, Morazain R, McGregor L. Effects of diet on blood clotting and platelet aggregation. In nutrition in the 1980s. Alan R Liss; New York: 1981. pp 361-381
- 10 Lagarde M, Burtin M, Sprecher H, Dechavanne M, Renaud S. Potentiating effect of 5,8,11-eicosatrienoic acid on human platelet aggregation. Lipids 1981; 18: 291-294
- 11 Nordoy A, Svensson B, Hoak J. The effects of albumin bound fatty acids on the platelet inhibitory function of human endothelial cells. Eur J Clin Invest 1979; 9: 5-10
- 12 Nordoy A, Svensson B. The simultaneous effect of albumin bound fatty acids on platelets and endothelial cells. Thrombos Res 1979; 15: 215-226
- 13 Lagarde M, Burtin M, Dechavanne M, Sicard B, Coiffier B. Dihomogammalinolenic acid (20 3ω6) is more anti-aggregatory than eicosapentaenoic (20 5ω3) in a platelet endothelial cell mixture. Prostaglandins Med 1980; 4: 177-183
- 14 Brox JH, Nordoy A. The effect of polyunsaturated fatty acids on endothelial cells and their production of prostacyclin, thromboxane and platelet inhibitory activity. Thromb Haemostas 1983; 50: 762-767
- 15 Spector AA, Kaduce TL, Figard PH, Norton KC, Hoak JC, Czervionke RL. Eicosapentaenoic acid and prostacyclin production by cultured human endothelial cells. J Lipid Res 1983; 24: 1595-1604
- 16 Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic arteria J Clin Invest 1973; 52: 2745-2756
- 17 Lagarde M, Sicard B, Guichardant M, Felisi O, Dechavanne M. Fatty acid composition in native and cultured human endothelial cells. In vitro 1984; 20: 33-37
- 18 Lagarde M, Drouot B, Guichardant M, Dechavanne M. In vitro incorporation and metabolism of some icosaenoic acids in platelets. Effect on arachidonic acid oxygenation Biochim Biophys Acta 1985; 833: 52-58
- 19 Lagarde M, Bryon PA, Guichardant M, Dechavanne M. A simple and efficient method for platelet isolation from their plasma. Thromb Res 1980; 17: 581-588
- 20 Siess W, Dray F. Very low levels of 6-keto prostaglandin F1α in human plasma. J Lab Clin Med 1982; 99: 388-398
- 21 Spector AA, Kaduce TL, Hoak JC, Fry GL. Utilisation of arachidonic and linoleic acids by cultured human endothelial cells. J Clin Invest 1981; 68: 1003-1011
- 22 Rosenthal MD, Whitehurst MC. Fatty acyl Δ6 desaturation activity of cultured human endothelial cells modulation by fetal bovine serum. Biochim Biophys Acta 1983; 750: 490-496
- 23 Croset M, Lagarde M. Stereospecific inhibition of PGH2 induced platelet aggregation by lipoxygenase products of icosaenoic acids. Biochem Biophys Res Commun 1983; 112: 878-883
- 24 Brotherton AA, Hoak JC. Prostacyclin biosynthesis in cultured vascular endothelium is limited by deactivation of cyclooxygenase. J Clin Invest 1983; 72: 1255-1261
- 25 Thomas JM, Hullen F, Chap H, Douste-Blazy L. Phosphatidyl choline is the major phospholipid providing arachidonic acid for prostacyclin synthesis in thrombin stimulated human endothelial cells. Thromb Res 1984; 34: 117-123
- 26 Dyerberg J, Jorgensen KA, Arnfred T. Human umbilical blood vessel converts all cis 5,8,11,14,17-eicosapentaenoic acid to prostaglandin I3 . Prostaglandins 1981; 22: 857-862
- 27 Homstra G, Christ-Hazelhof E, Haddeman E, Ten HoorF, Nugteren DH. Fish oil feeding lowers thromboxane and prostacyclin production by rat platelets and aorta and does not result in the formation of prostaglandin I3 . Prostaglandins 1981; 21: 727-738
- 28 Fischer S, Weber PC. Prostaglandin I3 is formed in vivo in man after dietary eicosapentaenoic acid. Nature 1984; 307: 165-168
- 29 Denning GM, Figard PH, Kaduce TL, Spector AA. Role of triglycerides in endothelial cell arachidonic metabolism. J Lipid Res 1983; 24: 993-1001