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Thromb Haemost 1985; 53(02): 268-272
DOI: 10.1055/s-0038-1661291
Original Article
Schattauer GmbH Stuttgart

Determination of Plasma Protein S - The Protein Cofactor of Activated Protein C

R M Bertina
The Haemostasis and Thrombosis Research Unit, Dept of Haematology, Leiden University Hospital, Leiden, The Netherlands
,
A van Wijngaarden
The Haemostasis and Thrombosis Research Unit, Dept of Haematology, Leiden University Hospital, Leiden, The Netherlands
,
J Reinalda-Poot
The Haemostasis and Thrombosis Research Unit, Dept of Haematology, Leiden University Hospital, Leiden, The Netherlands
,
S R Poort
The Haemostasis and Thrombosis Research Unit, Dept of Haematology, Leiden University Hospital, Leiden, The Netherlands
,
V J J Bom
The Haemostasis and Thrombosis Research Unit, Dept of Haematology, Leiden University Hospital, Leiden, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 18 October 1984

Accepted 06 February 1985

Publication Date:
18 July 2018 (online)

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Summary

Protein S, an important cofactor of activated protein C, and C4b-binding protein were purified from human plasma. Specific antibodies against the purified proteins were raised in rabbits and used for the development of immunologic assays for these proteins in plasma: an immunoradiometric assay for protein S (which measures both free protein S and protein S complexed with C4b-binding protein) and an electroimmunoassay for C4b- binding protein. Ranges for the concentrations of these proteins were established in healthy volunteers and patients using oral anticoagulant therapy. A slight decrease in protein S antigen was observed in patients with liver disease (0.78 ± 0.25 U/ml); no significant decrease in protein S was observed in patients with DIC (0.95 ± 0.25 U/ml).

Criteria were developed for the laboratory diagnosis of an isolated protein S deficiency

Keywords

Protein S - Activated protein C - C4b-binding protein - Thrombosis
 

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