Vitamin K Dependent Proteins in Fetal Hemostasis at Mid Trimester of Pregnancy

F Forestier; F Daffos; M Rainaut; Y Solé; J Amiral

doi:10.1055/s-0038-1661323

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1985; 53(03): 401-403
DOI: 10.1055/s-0038-1661323

Original Article

Schattauer GmbH Stuttgart

Vitamin K Dependent Proteins in Fetal Hemostasis at Mid Trimester of Pregnancy

F Forestier

The Centre de Diagnostic Prénatal et de Foetologie, Hôpital Notre Dame de Bon-Secours, Paris, France

,

F Daffos

The Centre de Diagnostic Prénatal et de Foetologie, Hôpital Notre Dame de Bon-Secours, Paris, France

,

M Rainaut

The Centre de Diagnostic Prénatal et de Foetologie, Hôpital Notre Dame de Bon-Secours, Paris, France

,

Y Solé

The Centre de Diagnostic Prénatal et de Foetologie, Hôpital Notre Dame de Bon-Secours, Paris, France

,

J Amiral

¹The Laboratoire STAGO, Franconville, France

› Author Affiliations

Abstract

PDF Download

Summary

The vitamin K dependent coagulation factor activities were measured in 63 normal human fetuses from 19 to 28 weeks of pregnancy. These activities were included between 9 to 28 percent of normal adult levels.

Prothrombin antigen, factor IX antigen and protein C were also measured. There is a good correlation between prothrombin procoagulant activity and antigenicity, suggesting that low level of these vitamin K dependent proteins in fetuses is probably a consequence of liver immaturity.

Keywords

Haemostasis - Fetus - Vitamin K

PDF (333 kb)

References

References
1 Daffos F, Capella-Pavlovsky M, Forestier F. Fetal blood sampling via the umbilical cord using a needle guided by ultrasound - Report of 66 cases. Prenat Diagn 1983; 3: 271-277
2 Daffos F, Capella-Pavlovsky M, Forestier F. A new procedure for fetal blood sampling in utero. Am J Obstet Gynecol 1984; 146: 985-987
3 Mackenzie IZ, MacLean OA. Pure fetal blood from the umbilical cord obtained by fetoscopy, experience with 125 consecutive cases. Am J Obstet Gynecol 1980; 138: 1214-1218
4 Daffos F, Forestier F, Keros-Grangeot L, Capella-Pavlovsky M, Lebon P, Chartier M, Pillot J. Prenatal diagnosis of congenital Rubella. Lancet 1984; 2: 1-3
5 Desmonts G, Daffos F, Forestier F, Thuilliez P, Capella-Pavlovsky M, Chartier M. Prenatal diagnosis of congenital toxoplasmosis. Lancet 1985; 1: 500-503
6 Forestier F, Daffos F, Capella-Pavlovsky M. Premier diagnostic prenatal d’une hémophilie A par ponction directe du cordon ombilical. Presse Méd 1983; 39: 2462-2463
7 Daffos F, Forestier F, Muller JY, Resnikoff-Etievant MF, Habibi B, Capella-Pavlovsky M, Maigret P, Kaplan C. Prenatal treatment of alloimmune thrombocytopenia. Lancet 1984; 2: 632
8 Mibashan RS, Rodeck CH, Thumpston JK, Edwards RS, Singer JD, White JM, Campbell S. Plasma assay of fetal factors VIIIc and IX for prenatal diagnosis of haemophilia. Lancet 1979; 1: 1309-1311
9 Bartlett A, Dormandy KM, Hawkey CM, Stableforth P, Voller A. Factor VIII related antigen: measurement by enzyme immunoassay. Br Med J 1976; 2: 259-267
10 Heremans JF, Masson PL. Specific analysis of immunoglobulins, technique and clinical value. Clin Chem 1973; 94: 84-86
11 Conrad A, Schumann J, Kreutz FM, Sieber A. Elaboration of a method for the quantitative determination of proteins by laser nephelometry in the clinical routine laboratory. J Clin Chem Clin Biochem 1978; 16: 299-301
12 Stenflo J. A new vitamin K dependent protein purification from bovine plasma and preliminary characterization. J Biol Chem 1976; 251: 355-363
13 Bertina RM, Broekmans AW, Van der Linden IK, Mertens K. Protein C deficiency in a Dutch family with thrombotic disease. Thromb Haemostas 1982; 48: 1-5
14 Hathaway WE, Bonnard J. Physiology of coagulation in the fetus and newborn infant. In Perinatal Coagulation. Grune & Stratton; New York: 1978. pp 53-80
15 Owen WG, Esmon CT. Functional properties of an endothelial cell cofactor for thrombin-catalyzed activation of protein C. J Biol Chem 1981; 256: 5532-5535
16 Kisiel W, Canfield WM, Ericson LH, Davie EW. Anticoagulant properties of bovine plasma protein C following activation by thrombin. Biochemistry 1977; 16: 5824-5831
17 Marlar RA, Kleiss AJ, Griffin JH. Anticoagulant action of human protein C. In Peetens H. (ed) Protides of the biological fluids. Vol.28. Pergamon Press; Oxford: 1980. pp 341-344
18 Camp PC, Esmon CT. Generation of fibrinolytic activity by infusion of activated protein C into dogs. J Clin Invest 1981; 68: 1221-1228
19 Griffin JH, Evatt B, Zimmermann TS, Kleiss AJ, Wideman C. Deficiency of protein C in congenital thrombotic disease. J Clin Invest 1981; 68: 1370-1373
20 Broekmans AW, Veltkamp JJ, Bertina RM. Congenital protein C deficiency and venous thromboembolism: a study of three Dutch families. N Engl J Med 1983; 309: 340-344
21 Pabinger-Fasching I, Bertina RM, Lechner K, Niessner H, Korninger C. Hereditary protein C deficiency in two Austrian families. Thromb Haemostas 1983; 50: 810-813
22 Horellou MH, Samama M, Conard J, Bertina RM. Protein C deficiency in 3 unrelated French patients with venous thrombosis. Thromb Haemostas 1983; 50: 351 (Abstr.)
23 Seligsohn U, Berger A, Abend M, Rubin L, Attias D, Zivelin A, Rapaport SI. Homozygous protein C deficiency manifested by massive venous thrombosis in the newborn. N Engl J Med 1984; 310: 599-662
24 Estelles A, Garcia Plaza I, Dasi A, Aznar J, Duart M, Sanz G, Perez Requejo J, Espana F, Jimenez C, Abeledo G. Severe inherited “homozygous” protein C deficiency in a newborn infant. Thromb Haemostas 1984; 52: 53-56
25 Stenflo J, Ferlund P, Egan W, Roepstorff P. Vitamin K dependent modifications of glutamic acid residues in prothrombin. Proc Natl Acad Sci USA 1974; 71: 2730-2733
26 Nelsestuen GL, Zytkovic TH, Howard JB. The mode of action of vitamin K: Identification of carboxyglutamic acid as a component of prothrombin. J Biol Chem 1974; 249: 6347-6350
27 Magnusson S, Sottrup-Jensen L, Petersen TE, Morris HR, Dew A. Primary structure of vitamin K dependent part of prothrombin. FEBS Lett 1974; 44: 189-193
28 Friedman PA. Vitamin K dependent proteins. N Engl J Med 1984; 310: 1458-1460
29 Esmon CT, Suttie JW, Jackson CM. The functional significance of vitamin K action: difference of phospholipid binding between normal and abnormal prothrombin. J Biol Chem 1975; 250: 4095-4099
30 Suttie JW. Mechanism of action of vitamin K: Demonstration of a liver precursor of prothrombin. Science 1973; 179: 192-193
31 Hemker HC, Veltkamp JJ, Loeliger EA. Kinetic aspects of the interaction of blood clotting enzymes. III. Demonstration of an inhibitor of prothrombin conversion in vitamin K deficiency. Thromb Haemostas 1968; 19: 346-363
32 Hall IG, Pauli RM, Wilson KM. Maternal and foetal sequel of anticoagulation during pregnancy. Am J Med 1980; 68: 122-140
33 Davies VA, Rothberg AD, Argent AC, Atkinson PM, Staub H, Pienaar NL. Precursor prothrombin status in patients receiving anticonvulsant drugs. Lancet 1985; 1: 126-128
34 Malia RG, Preston FE, Mitchell VE. Evidence against vitamin K deficiency in normal neonates. Thromb Haemostas 1980; 44: 159-160