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Thromb Haemost 1986; 55(01): 040-046
DOI: 10.1055/s-0038-1661443
Original Article
Schattauer GmbH Stuttgart

The Production and Characterisation of a Panel of Ten Murine Monoclonal Antibodies to Human Procoagulant Factor VIII

B D Griffin
1   The Scottish National Blood Transfusion Service Headquarters Unit Laboratory, Edinburgh, Scotland, UK
,
L R Micklem
2   The Department of Surgery, University of Edinburgh Medical School, Edinburgh, Scotland, UK
,
M C McCann
2   The Department of Surgery, University of Edinburgh Medical School, Edinburgh, Scotland, UK
,
K James
2   The Department of Surgery, University of Edinburgh Medical School, Edinburgh, Scotland, UK
,
D S Pepper
1   The Scottish National Blood Transfusion Service Headquarters Unit Laboratory, Edinburgh, Scotland, UK
› Author Affiliations
Further Information

Publication History

Received 05 August 1985

Accepted 14 October 1985

Publication Date:
19 July 2018 (online)

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Summary

A panel of 10 murine monoclonal antibodies to procoagulant FVIII has been developed from the fusion of a single spleen. Balb/c mice were injected with a purified preparation of FVIII: Ag, and antibody production in sera and hybrid culture supernatants was monitored using a specific radiometric screening assay. The antibodies all inhibit FVIII clotting activity in normal plasma, and when immobilised on agarose retain their ability to recognise and bind the FVIII procoagulant protein. Studies on protein A-purified immunoglobulins demonstrate a range of properties within the panel of antibodies with regard to species crossreactivity, clotting inhibition and immunoadsorption. The panel of antibodies has been used to screen heat-treated FVIII concentrates for the occurrence of heat-induced neoantigens.

Key words

Factor VIII - Monoclonal antibodies
 

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