Subscribe to RSS
DOI: 10.1055/s-0038-1661450
Evaluation of the Cuticle Bleeding Time in Canine Haemophilia A
Publication History
Received 06 September 1985
Accepted 31 October 1985
Publication Date:
19 July 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/198601/lookinside/thumbnails/10-1055-s-0038-1661450-1.jpg)
Summary
In this paper we describe our clinical experience and results with the cuticle bleeding time test in a colony of cross-bred Labrador retrievers with severe haemophilia A. The dogs have a severe bleeding tendency with a high incidence of fatal haemorrhages in the central nervous system. Homozygous females appeared to be especially prone to this lethal complication. Factor VIII recovery and half-life determinations yielded results similar to the data from human studies. The cuticle bleeding time proved to be a good measure of the coagulation defect. The prolongation of the bleeding time could be completely abolished by administration of 10 to 15 units of canine factor VIII per kg body weight. We conclude that the cuticle bleeding time in canine haemophilia provides us with a suitable model for the in vivo study of new therapeutic materials.
-
References
- 1 Luscher JM, Shapiro SS, Palascak JE, Rao AV, Levine OH, Blatt PM. The Hemophilia Study Group. Efficacy of prothrombin-complex concentrates in hemophiliacs with antibodies to factor VIII. N Engl J Med 1980; 303: 421-425
- 2 Sjamsoedin LJM, Heijnen L, Mauser-Bunschoten EP, Van Geijlswijk JL, Van Houwelingen H, Van Asten P, Sixma JJ. The effect of activated prothrombin-complex concentrate (FEIBA) on joint and muscle bleeding in patients with hemophilia A and antibodies to factor VIII. N Engl J Med 1981; 305: 717-721
- 3 Luscher JM, Blatt PM, Penner JA, Aledort LM, Levine PH, White GC, Warrier AI, Whitehurst DA. Autoplex versus proplex: a controlled, double-blind study of effectiveness in acute hemarthroses in hemophiliacs with inhibitors to factor VIII. Blood 1983; 62: 1135-1138
- 4 Giles AR, Tinlin S, Greenwood R. A canine model of hemophilic (factor VIII :C deficiency) bleeding. Blood 1982; 60: 727-731
- 5 Kingdon HS, Hassell TM. Hemophilic dog model for evaluating therapeutic effectiveness of plasma protein fractions. Blood 1981; 58: 868-873
- 6 Owren PA. A quantitative one-stage method for the assay of prothrombin. Scand J Lab Invest 1949; 1: 81-83
- 7 Veltkamp JJ, Asfaou E, Van de Torren K, Van der Does JA, Van Tilburg NH, Pauwels EKJ. Extrahepatic factor VIII synthesis. Lung transplants in hemophilic dogs. Transplantation 1974; 18: 56-62
- 8 Kasper CK, Aledort LM, Counts RB, Edson JR, Fratantoni J, Green D, Hampton JW. et al. A more uniform measurement of factor VIII inhibitors. Thrombos Diathes Haemorrh 1975; 34: 869-872
- 9 Kjellman H. Calculations of factor VIII in vivo recovery and half-life. Scand J Haematol 1984; 33 Suppl (Suppl. 40) 165-174
- 10 Lewis JH, Spero JA, Hasiba U. A hemophilic dog colony: genetic studies and coagulation findings in hemophiliac and normal dogs. Comp Biochem Physiol 1983; 75A: 147-151