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Thromb Haemost 1986; 55(02): 246-249
DOI: 10.1055/s-0038-1661530
Original Article
Schattauer GmbH Stuttgart

Electroblot and Immunoperoxidase Staining for Rapid Screening of the Abnormalities of the Multimeric Structure of von Willebrand Factor in von Willebrand’s Disease

Rossana Lombardi
*   The A. Bianchi Bonomi Hemophilia and Thrombosis Center, Institute of Internal Medicine, University of Milan, Italy
,
Cecilia Gelfi
**   The University of Milan and the Chair of Biochemistry, Faculty of Pharmacy and Department of Biomedical Sciences and Technology, University of Milan, Italy
,
P G Righetti
**   The University of Milan and the Chair of Biochemistry, Faculty of Pharmacy and Department of Biomedical Sciences and Technology, University of Milan, Italy
,
Antonella Lattuada
*   The A. Bianchi Bonomi Hemophilia and Thrombosis Center, Institute of Internal Medicine, University of Milan, Italy
,
P M Mannucci
*   The A. Bianchi Bonomi Hemophilia and Thrombosis Center, Institute of Internal Medicine, University of Milan, Italy
› Author Affiliations
Further Information

Publication History

Received 06 December 1985

Accepted 17 February 1986

Publication Date:
18 July 2018 (online)

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Summary

A new electrophoretic method is described for rapid screening of abnormalities of the multimeric structure of von Willebrand factor in von Willebrand’s disease. The method is based on the transfer of the separated proteins from agarose gels onto nitrocellulose foils followed by immunoperoxidase staining. It has the advantage of not requiring radio-iodinated antibodies and reduces the working time for the entire procedure from 5-6 days to 3 days. Electroblotting followed by immunoperoxidase staining differentiates patients with intact multimeric structure from those without intermediate and/or large multimers. The more subtle defects of the inner structure of the smallest multimers found in patients with type II von Willebrand’s disease can also be identified. A potential disadvantage of electroblotting and immunoperoxidase staining is the lesser sensitivity of this technique, which results in the detection of a smaller number of multimers (11-12 bands) than by autoradiography without transfer onto nitrocellulose (16-17 bands).

Key words

Electroblots - von Willebrand Factor - von Willebrand’s Disease - Immunoperoxidase
 

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