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Thromb Haemost 1986; 56(01): 035-039
DOI: 10.1055/s-0038-1661598
Original Article
Schattauer GmbH Stuttgart

Absence of Synergism Between Tissue-Type Plasminogen Activator (t-PA), Single-Chain UrokinaseType Plasminogen Activator (scu-PA) and Urokinase on Clot Lysis in a Plasma Milieu In Vitro

D Collen
The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
,
F De Cock
The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
,
E Demarsin
The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
,
H R Lijnen
The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
,
D C Stump
The Center for Thrombosis and Vascular Research, University of Leuven, Belgium
› Author Affiliations
Further Information

Publication History

Received 07 February 1986

Accepted 15 May 1986

Publication Date:
13 July 2018 (online)

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Summary

A potential synergic effect of tissue-type plasminogen activator (t-PA), single-chain urokinase-type plasminogen activator (scuPA) or urokinase on clot lysis was investigated in a whole human plasma system in vitro. The system consisted of a human plasma clot labeled with 125I-fibrinogen, immersed in titrated whole human plasma, to which the thrombolytic agents were added. Clot lysis was quantitated by measurement of released 125I, and activation of the fibrinolytic system in the surrounding plasma by measurements of fibrinogen and α2-antiplasmin.

t-PA, scu-PA and urokinase induced a dose-dependent and time-dependent clot lysis; 50 percent lysis after 2 h was obtained with 5 nM t-PA, 20 nM scu-PA and 12 nM urokinase. At these concentrations no significant activation of the fibrinolytic system in the plasma was observed with t-PA and scu-PA, whereas urokinase caused significant α2-antiplasmin consumption and concomitant fibrinogen degradation. The shape of the dose-response curves was different; t-PA and urokinase showed a log linear dose-response whereas that of scu-PA was sigmoidal.

Keywords

t-PA - scu-PA - Urokinase - Synergism - Thrombolysis
 

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