Summary
Collagen (10-40 μg kg−1), thrombin (1-10 units kg−1), adenosine diphosphate (ADP; 3-300 pμ kg−1), 1-0-hexadecyl Paf-acether and 1-0-octadecyl Paf-acether (1-300 ng kg−1) administered by bolus intravenous injection each caused dose-dependent thrombocytopoenia
accompanied by marked hypotension in anaesthetized rabbits. Responses to ADP and the
Paf-acether derivatives were transient in nature (3-8 min) whereas those induced by
collagen and thrombin were always of longer duration (5-20 min) and frequently fatal
at high doses. Responses to collagen, thrombin, and the Paf-acether derivatives were
invariably accompanied by substantial, dose-related increases in plasma levels of
thromboxane B2 in samples obtained 30 s after agonist administration, whereas following ADP, no
change in plasma thromboxane B2 was detected at any dose level. Indomethacin (3.0 mg kg−1 by infusion) had no effect on responses to thrombin or Paf-acether, partially inhibited
collagen-induced thrombocytopenia, and potentiated responses to ADP. In contrast,
dazoxiben (10 mg kg−1 by infusion) partially but significantly inhibited responses to thrombin, whereas
those induced by collagen, Paf-acether or ADP were unchanged. These results indicate
that in this model of intravascular aggregation, whilst platelet responses to collagen
and thrombin appear partially dependent on intact cyclic endoperoxide and thromboxane
A2 synthetic capacity respectively, responses to ADP and Paf-acether are independent
of arachidonate metabolism via cyclo-oxygenase despite measurably increased TXB2 formation in the latter case.
Keywords
Rabbit intravascular aggregation - Indomethacin - Dazoxiben
