Thromb Haemost 1986; 56(01): 090-094
DOI: 10.1055/s-0038-1661609
Original Article
Schattauer GmbH Stuttgart

Role of Heparin in Tumor Cell-Induced Platelet Aggregation

Kazuo Yamamoto
The Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
,
Hisayo Kitagawa
The Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
,
Kenjiro Tanoue
The Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
,
Takashi Tsuruo
1   The Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Tokyo, Japan
,
Naomasa Yamamoto
The Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
,
Hiroh Yamazaki
The Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
› Author Affiliations
Further Information

Publication History

Received 17 March 1986

Accepted 09 June 1986

Publication Date:
13 July 2018 (online)

Preview

Summary

B16 mouse melanoma cell lines (B16F1, B16F10 and B16BL6) were able to induce platelet aggregation, and concomitant release of ATP in heparinized platelet-rich plasma (PRP). In citrated PRP, these tumor cells did not induce platelet aggregation. Addition of heparin to citrated PRP enabled these tumor cells to induce aggregation. In heparinized PRP, platelet aggregates induced by B16F10 cells were dissociated by the addition of either 4 mM EDTA, 10 mM CaCl2 or 0.1 μg/ml protamine sulfate. B16F10-induced aggregation in heparinized PRP was inhibited by preincubation with anti-fibronectin antibody, but not with antifibrinogen or anti-von Willebrand factor antibodies. B16F10 cells induced aggregation in washed platelet suspension with the addition of heparinized platelet-poor plasma (PPP). Cryoprecipi-tate from human plasma showed the same effect in the presence of heparin if substituted for PPP. The mixture of purified fibronectin, von Willebrand factor, fibrinogen and heparin were less effective than cryoprecipitate on B16F10-induced aggregation of washed platelets. The results suggest that an interaction between fibronectin and heparin may be important in tumor cell-induced aggregation.