Electrical Aggregometry in Whole Blood from Human, Pig and Rabbit

Angel E Galvez; Lina Badimon; Juan-Jose Badimon; Valentin Fuster

doi:10.1055/s-0038-1661625

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1986; 56(02): 128-132
DOI: 10.1055/s-0038-1661625

Original Article

Schattauer GmbH Stuttgart

Electrical Aggregometry in Whole Blood from Human, Pig and Rabbit

Authors

Angel E Galvez

The Division of Cardiology, Mount Sinai Medical Center, New York, New York, USA
Lina Badimon

The Division of Cardiology, Mount Sinai Medical Center, New York, New York, USA
Juan-Jose Badimon

The Division of Cardiology, Mount Sinai Medical Center, New York, New York, USA
Valentin Fuster

The Division of Cardiology, Mount Sinai Medical Center, New York, New York, USA

Abstract

PDF Download

Summary

The objective of this study was to characterize and standardize whole blood electrical aggregometry (WBEA) in the pig and rabbit, animal models extensively used in atherosclerosis research, and to compare their platelet response with that of man. Platelet aggregation was studied in blood (WBEA) and platelet rich plasma (optical aggregometry, OA). Dose response curves were obtained for ADP and collagen. The effect of hematocrit on WBEA was also evaluated. Aggregation with ADP and collagen using WBEA was more extensive with human than with pig or rabbit platelets. OA revealed similar differences among species but the time to reach maximal aggregation was markedly shorter. Using WBEA, the extent of aggregation was inversely related to the hematocrit. We conclude that WBEA is a useful technique that may be of particular importance in situations where hyper-lipidemic plasma prevents the use of OA, as occurs in some atherosclerosis research animal models.

Key words

Electrical aggregometry - Platelets - Animal models - Whole blood aggregation

PDF (137 kb)

References

References
1 Cardinal DC, Flower RJ. The electronic aggregometer: a novel device for assessing platelet behavior in blood. J Pharm Meth 1980; 3: 135-158
2 Russell-Smith NC, Flower RJ, Cardinal DC. Measuring platelet and leukocyte aggregation/adhesion responses in very small volumes of whole blood. J Pharm Meth 1981; 6: 315-333
3 Blackwell GJ, Flower RJ, Russell-Smith N, Salmon JA, Thorogood PB, Vane JR. Prostacyclin is produced in whole blood. Br J Pharmacol 1978; 64: 436
4 Gaarder A, Jonsen J, Laland S, Hellem A, Owren PA. Adenosine diphosphate in red cells as a factor in the adhesiveness of human blood platelets. Nature 1961; 192: 531-532
5 Ross H, Pfleger K. Kinetics of adenosine uptake by erythrocytes and the influence of dipyridamol. Mol Pharmacol 1972; 8: 417-425
6 Gresele P, Zoja C, Deckmyn H, Arnout J, Vermylen J, Verstraete M. Dipyridamole inhibits platelet aggregation in whole blood. Thromb Haemostas 1983; 50: 852-856
7 Chien S, Gregersen M. Physical techniques in biological research. Nastuk WL. (Ed.) Academic Press; New York: 1962
8 Born G VR. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature 1962; 194: 927-929
9 Ingerman-Wojensky C, Smith JB, Silver MJ. Evaluation of electrical aggregometry: comparison with optical aggregometry, secretion of ATP, and accumulation of radiolabeled platelets. J Lab Clin Med 1983; 101: 44-52
10 Fox SC, Burgess-Wilson M, Heptinstall S, Mitchell J RA. Platelet aggregation in whole blood determined using the Ultra-Flo 100 platelet counter. Thromb Haemostas 1982; 48: 327-329
11 Saniabadi AR, Lowe G DO, Forbes CD, Prentice CRM, Barbenel JC. Platelet aggregation studies in whole human blood. Thromb Res 1983; 30: 625-632
12 Cheeseman JE, Mills SP, Hardisty RM. Platelet aggregometry on whole blood: the use of the ELT 8/ds blood cell counter in the investigation of bleeding disorders. Clin Lab Haemat 1984; 6: 265-272
13 Challen A, Branch WJ, Cummings JH. Quantitation of platelet mass during aggregation in the electronic (Wellcome) whole blood aggregometer. J Pharm Meth 1982; 8: 115-122
14 Baumgartner H. The role of blood flow in platelet adhesion, fibrin deposition, and formation of mural thrombi. Microvasc Res 1973; 5: 167-179
15 Badimon L, Badimon JJ, Galvez A, Chesebro JA, Fuster V. Influence of arterial damage and wall shear rate on platelet deposition, ex vivo study in a swine model. Arteriosclerosis 1986; 6: 312-320
16 Regent N, Born G VR. Growth rate in vivo of platelet thrombi, produced by iontophoresis of ADP, as a function of mean blood flow velocity. Nature 1970; 227: 926-930
17 Richardson PD. Effect of blood flow velocity on growth rate of platelet thrombi. Nature 1973; 245: 103-104
18 Machi J, Sigel B, Ramos JR, Justin JR, Feinberg H, LeBreton GC, Robertson AL. Role of red cells in preventing the growth of platelet aggregation. Thromb Res 1984; 36: 53-66
19 Mackie IJ, Jones R, Machin SJ. Platelet impedance aggregation in whole blood and its inhibition by antiplatelet drugs. J Clin Pathol 1984; 37: 874-878
20 Mills D CB. Platelet aggregation and platelet nucleotide concentration in different species. Symp Zool Soc 1970; 27: 99-107
21 Macmillan DC, Sim AK. A comparative study of platelet aggregation in man and laboratory animals. Thromb Diath Haem 1970; 24: 385-394
22 Kinlough-Rathbone RL, Cazenave JP, Packham MA, Mustard JF. Effect of inhibitors of the arachidonate pathway on the release of granule contents from rabbit platelets adherent to collagen. Lab Invest 1980; 42: 28-34
23 Packham MA, Mustard JF. Pharmacology of platelet-affecting drugs. Circulation 1980; 62 (Suppl. 05) V-26
24 Badimon JJ, Galvez A, Badimon L, Mao S, Fuster V. High density lipoproteins and experimental rabbit arterial disease. Thromb Haemostas 1985; 54: 86 (Abstr)