Summary
Endothelial cells were cultured from various human arteries and veins, obtained from
adult individuals and from umbilical cords. We compared the storage and secretion
of von Willebrand factor by endothelial cells from umbilical veins with that of endothelial
cells cultured from a number of adult vessels, including aorta, arteria iliaca, vena
saphena magna and vena cava. There were no differences in the way the cultured endothelial
cells handled the von Willebrand factor they synthesized. Endothelial cells from the
various vessels responded to stimuli in secreting stored von Willebrand factor. The
cells also responded to thrombin and ionophore A23187 in producing enhanced amounts
of prostacyclin. Thus, cultured umbilical vein endothelial cells have properties that
are very similar to those of cultured endothelial cells of various other origins.
It is concluded that foetal venous cells provide a representative model for studies
of endothelial cell von Willebrand factor biosynthesis and prostacyclin production.
Key words
(Human) Endothelial cells - von Willebrand factor - Prostacyclin
