Heparin-Induced Osteoporosis in Rats

Thomas Matzsch; David Bergqvist; Ulla Hedner; Bo Nilsson; Per Østergaard

doi:10.1055/s-0038-1661669

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1986; 56(03): 293-294
DOI: 10.1055/s-0038-1661669

Original Article

Schattauer GmbH Stuttgart

Heparin-Induced Osteoporosis in Rats

Thomas Matzsch

^*The Department of General Surgery, University of Lund, Malmö General Hospital, Sweden

,

David Bergqvist

^*The Department of General Surgery, University of Lund, Malmö General Hospital, Sweden

,

Ulla Hedner

^**The Novo Research Institute, Copenhagen, Denmark

,

Bo Nilsson

^***The Department of Orthopedics, University of Lund, Malmö General Hospital, Sweden

,

Per Østergaard

^**The Novo Research Institute, Copenhagen, Denmark

› Author Affiliations

Abstract

PDF Download

Summary

In order to study the effect of heparin in inducing osteoporosis, 30 female rats were divided in two groups and treated with daily injections of 2 IU heparin/g body weight for 33 and 65 days and compared with the same number of rats acting as controls. The mineral bone mass in the femora of the animals was measured quantitatively. A significant (p <0.001) reduction in bone mineral mass was found in the heparin-treated animals. This effect was present to the same degree after 33 days as after 65 days of treatment. It is concluded that heparin in this dose causes osteoporosis in rats after 33 days and that the described method can be used as an experimental model for further studies on this topic.

Keywords

Heparin - Osteoporosis

PDF (405 kb)

References

References
1 Griffith GC, Nichols G, Asher JD, Flanagan B. Heparin Osteoporosis. JAMA 1965; 193: 85-88
2 Ringe J-D, Becker K. Osteoporoseentwicklung bei Heparinlangzeit-therapie. Med Mo Pharm 1985; 8: 80-83
3 Gudmundson C, Nilsson BE. Failure to produce heparin osteoporosis in rats. Acta Pharmacol Toxicol 1969; 27: 56-59
4 Hähnel H, Lindenhayn K, Mühlbach R, Schmidt UJ. Die Heparin-osteopathie im Tierexperiment - ein Osteoporosemodell?. Z Alterns-forsch 1973; 27: 71-75
5 Smith JK, Case MT. Effect of long-term heparin administration in dogs. Drug Chem Toxicol 1978; 51: 251-258
6 Thompson RC. Heparin Osteoporosis. An experimental model using rats. J Bone Joint Surg 1973; 55A: 606-612
7 Asher JD, Nichols G. Heparin stimulation of bone collagenase activity. Fed Proc 1965; 24: 211
8 Jaffe MD, Willis PW. Multiple fractures associated with long-term sodium heparin therapy. JAMA 1965; 193: 152-154
9 Aarskog D, Aksnes L, Lehmann V. Low 1,25-Dihydroxyvitamin D in heparin-induced osteopenia. Lancet 1980; 2: 650-651
10 Avioli LV. Heparin-induced osteopenia: an appraisal. Adv Exp Med Biol 1975; 52: 375-387
11 Cryer PE, Kissane JM. Systemic mastocytosis. Amer J Med 1976; 61: 671-680
12 Sackler JP, Liu L. Heparin-induced osteoporosis. Br J Radiol 1973; 46: 548-550
13 Hellgren M, Nygards E-B. Long-term therapy with subcutaneous heparin during pregnancy. Gynecol Obstet Invest 1982; 13: 76-89
14 Howell R, Fidler J, Letsky E. The risks of antenatal subcutaneous heparin prophylaxis; a controlled trial. Br J Obstet Gynaecol 1983; 90: 1124-1128
15 Rupp WM, McCarthy HB, Rohde TD, Blackshear PJ, Phil D, Goldenberg FJ, Buchwald H. Risk of osteoporosis in patients treated with long-term intravenous heparin therapy. Curr Surg 1982; 39: 419-422
16 De Swiet M, Ward PD, Fidler J, Horsman A, Katz D, Letsky E, Peacock M, Wise PH. Prolonged heparin therapy in pregnancy causes bone demineralization. Br J Obstet Gynaecol 1983; 90: 1129-1134
17 Griffiths HT, Liu DT Y. Severe heparin osteoporosis in pregnancy. Postgrad Med J 1984; 60: 424-425