Functional and Antigenic Antithrombin III Values in Plasma and Serum from 115 Regular Blood Donors and Antithrombin III In Prediction of Postoperative Thrombosis

D W T Nilsen; M Jeremic; O K Weisert; E Fønstelien; H C Godal

doi:10.1055/s-0038-1665247

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1983; 50(02): 530-533
DOI: 10.1055/s-0038-1665247

Original Article

Schattauer GmbH Stuttgart

Functional and Antigenic Antithrombin III Values in Plasma and Serum from 115 Regular Blood Donors and Antithrombin III In Prediction of Postoperative Thrombosis

D W T Nilsen

The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway

,

M Jeremic

The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway

,

O K Weisert

The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway

,

E Fønstelien

The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway

,

H C Godal

The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway

› Author Affiliations

Abstract

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Summary

Antithrombin III (AT-III) was determined functionally using chromogenic substrate (S-2238) and immunologically using radial immunodiffusion (RID) in plasma and serum from 115 blood donors. There was a decrease in functional activity by 43.5% and in antigen concentration by 18.4% during in vitro coagulation when corresponding serum and plasma samples were compared. A positive correlation was found between the two methods in plasma (r=0.784) as well as in serum (r = 0.658). RID in serum correlated well with RID in plasma (r = 0.811), but the correlation for S-2238 in plasma and serum was poor (r = 0.411). Functional AT-III decrease during coagulation was uncorrelated with age and fibrinogen, and was not statistically affected by sex, smoking or blood groups.

Preoperative functional AT-III activity was measured in 25 patients undergoing elective hip replacement, 60% of whom developed postoperative thrombosis. The functional activity in serum discriminated well between the thrombotic and nonthrombotic group of patients (p<0.025), whereas the activity in plasma showed only a minor difference.

Keywords

Plasma-antithrombin III - Serum-antithrombin III - Post-operative thrombosis

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References

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