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Thromb Haemost 1983; 50(02): 530-533
DOI: 10.1055/s-0038-1665247
Original Article
Schattauer GmbH Stuttgart

Functional and Antigenic Antithrombin III Values in Plasma and Serum from 115 Regular Blood Donors and Antithrombin III In Prediction of Postoperative Thrombosis

D W T Nilsen
The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway
,
M Jeremic
The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway
,
O K Weisert
The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway
,
E Fønstelien
The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway
,
H C Godal
The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway
› Author Affiliations
Further Information

Publication History

Received 04 January 1983

Accepted 18 May 1983

Publication Date:
18 July 2018 (online)

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Summary

Antithrombin III (AT-III) was determined functionally using chromogenic substrate (S-2238) and immunologically using radial immunodiffusion (RID) in plasma and serum from 115 blood donors. There was a decrease in functional activity by 43.5% and in antigen concentration by 18.4% during in vitro coagulation when corresponding serum and plasma samples were compared. A positive correlation was found between the two methods in plasma (r=0.784) as well as in serum (r = 0.658). RID in serum correlated well with RID in plasma (r = 0.811), but the correlation for S-2238 in plasma and serum was poor (r = 0.411). Functional AT-III decrease during coagulation was uncorrelated with age and fibrinogen, and was not statistically affected by sex, smoking or blood groups.

Preoperative functional AT-III activity was measured in 25 patients undergoing elective hip replacement, 60% of whom developed postoperative thrombosis. The functional activity in serum discriminated well between the thrombotic and nonthrombotic group of patients (p<0.025), whereas the activity in plasma showed only a minor difference.

Keywords

Plasma-antithrombin III - Serum-antithrombin III - Post-operative thrombosis
 

  • References

  • 1 Abildgaard U. Antithrombin and related inhibitors of coagulation. In Recent advances in blood coagulation. Poller L. (ed) Churchill Livingstone; Edinburgh - London: 1981. pp 151-173
    Search in Google Scholar
  • 2 Abildgaard U. Binding of thrombin to antithrombin III. Scand J Clin Invest 1969; 24: 23-27
    CrossrefPubMedSearch in Google Scholar
  • 3 Yin ET, Wessler S, Stoll PJ. Identity of plasma-activated factor X inhibitor with antithrombin III and heparin cofactor. J Biol Chem 1971; 246: 3712-3719
    PubMedSearch in Google Scholar
  • 4 Rosenberg RD. Actions and interactions of antithrombin and heparin. N Engl J Med 1975; 292: 146-151
    CrossrefPubMedSearch in Google Scholar
  • 5 Godal HC, Rygh M, Laake K. Progressive inactivation of purified factor VII by heparin and antithrombin III. Thromb Res 1974; 5: 773-775
    CrossrefPubMedSearch in Google Scholar
  • 6 Mancini G, Carbonara AO, Hereman JF. Immunochemical quantitation of antigens by single radial immunodiffusion. Int J Immunochem 1965; 2: 235-254
    CrossrefPubMedSearch in Google Scholar
  • 7 Laurell CB. Quantitative estimations of proteins by electrophoresis in agarose gel containing antibodies. Ann Biochem 1966; 15: 45-52
    CrossrefPubMedSearch in Google Scholar
  • 8 Kaulla vonE, Kaulla vonK. Antithrombin III and diseases. Am J Clin Pathol 1967; 46: 69-80
    PubMedSearch in Google Scholar
  • 9 Abildgaard U, Fagerhol MK, Egeberg O. Comparison of progressive antithrombin activity and the concentrations of three thrombin inhibitors in human plasma. Scand J Clin Lab Invest 1970; 26: 349-354
    CrossrefPubMedSearch in Google Scholar
  • 10 Blombäck M, Blombäck B, Olsson P, Svendsen L. The assay of antithrombin using a synthetic chromogenic substrate for thrombin. Thromb Res 1974; 5: 621-632
    CrossrefPubMedSearch in Google Scholar
  • 11 Ødegaard OR, Lie M, Abildgaard U. Heparin cofactor activity measured with an amidolytic method. Thromb Res 1975; 6 (04) 287-294
    CrossrefPubMedSearch in Google Scholar
  • 12 McLellan DS, Devlin JD, Heyse-Moore GH, Aronstam A. Comparison of three methods for the estimation of plasma antithrombin. J Clin Pathol 1980; 33: 438-444
    CrossrefPubMedSearch in Google Scholar
  • 13 King PG, Huang AH, Palkuti HA, Farris VL. An evaluation of antithrombin III laboratory tests. Am J Clin Pathol 1980; 73: 537-540
    CrossrefPubMedSearch in Google Scholar
  • 14 Honegger H, Anderson N, Hewitt LA, Tullis JL. Antithrombin III profiles in malignancy, relationship to primary tumors and metastatic sites. Thromb Haemostas 1981; 46: 500-503
    PubMedSearch in Google Scholar
  • 15 Nilsen DW T, Jeremic M, Weisert OK. An attempt at predicting postoperative deep vein thrombosis by preoperative coagulation studies in patients undergoing total hip replacement. Thromb Haemostas 1980; 43: 194-197
    PubMedSearch in Google Scholar
  • 16 Weisert O, Jeremic M. Plasma fibrinogen levels in 1.016 regular blood donors. I. The influence of age and sex on mean values and percentiles. Vox Sang 1974; 27: 176-185
    CrossrefPubMedSearch in Google Scholar
  • 17 Kakkar VV. The diagnosis of deep vein thrombosis using the125I Fibrinogen test. Arch Surg 1972; 104: 152-159
    CrossrefPubMedSearch in Google Scholar
  • 18 McKay EJ. Immunochemical analysis of active and inactive antithrombin III. Br J Haematol 1980; 46: 277-285
    CrossrefPubMedSearch in Google Scholar
  • 19 Fagerhol MK, Abildgaard U. Immunochemical studies on human antithrombin III. Influence of age, sex and use of oral contraceptives on serum concentration. Scand J Haematol 1970; 7: 10-17
    PubMedSearch in Google Scholar
  • 20 Ødegaard OR, Teien AN. Antithrombin III, heparin cofactor and antifactor Xa in a clinical material. Thromb Res 1976; 8: 173-178
    CrossrefPubMedSearch in Google Scholar
  • 21 Innerfield I, Stone ML, Mersheimer W, Clauss RD, Greenberg J. Antithrombin and heparin antithrombin patterns in prethrombosis and thrombosis. J Clin Pathol 1976; 65: 384-389
    CrossrefPubMedSearch in Google Scholar
  • 22 Innerfield I, Goldfischer JD, Reicher-Reitt H, Greenberg J. Serum antithrombin III in coronary-artery disease. Am J Clin Pathol 1976; 65: 64-68
    CrossrefPubMedSearch in Google Scholar
  • 23 Winter JH, Bennett B, McTaggart F, Douglas AS. Lipoprotein fractions and antithrombin III consumption during clotting. Thromb Haemostas 1982; 47: 236-238
    PubMedSearch in Google Scholar