Adhesion and Aggregation of Thrombin Prestimulated Human Platelets on Surface-Bound Fibrinogen: Evidence for Involvement of ADP and Arachidonic Acid Pathways

I A Feuerstein; V M Dickson

doi:10.1055/s-0038-1665287

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1983; 50(03): 679-685
DOI: 10.1055/s-0038-1665287

Original Article

Schattauer GmbH Stuttgart

Adhesion and Aggregation of Thrombin Prestimulated Human Platelets on Surface-Bound Fibrinogen: Evidence for Involvement of ADP and Arachidonic Acid Pathways

I A Feuerstein

The Departments of Pathology and Chemical Engineering, McMaster University, Hamilton, Ontario, Canada

,

V M Dickson

The Departments of Pathology and Chemical Engineering, McMaster University, Hamilton, Ontario, Canada

› Author Affiliations

Abstract

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Summary

The purpose of this study was to examine the influence of substances released from human platelets upon their accumulation on human fibrinogen-coated glass tubes. After prestimulation with thrombin for one minute or in the absence of prestimulation, washed human platelets suspended in Eagle’s medium with RBC were drawn through the tubes at 1 ml/min, 80 s^-1, for 1, 2 or 6 min. Thrombin prestimulation (0.02,0.05 or 0.25 U/ml) was followed by inactivation with hirudin (0.1, 0.25 or 1.25 U/ml) before flow. Singly adherent platelets were observed in the absence of thrombin or with thrombin for exposure times of 1 and 2 min. At 6 min after at least 0.05 U/ml of thrombin, surface- bound aggregates were observed. The initial rate of adhesion increased with the amount of thrombin used for prestimulation. For adhesion to fibrinogen in the absence of prestimulation, platelet-derived ADP was a stimulator. Adhesion was shown to be independent of the ADP and arachidonic acid pathways in response to prestimulation with a low level of thrombin, 0.02 U/ml. For adhesion and cohesion, aggregation, in the presence of sufficient thrombin for prestimulation, 0.05 U/ml, ADP, serotonin and substances from arachidonic acid metabolism acted jointly to stimulate platelets.

Keywords

Platelet adhesion - Platelet aggregation - Fibrinogen-binding - Thrombin

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References

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