Thromb Haemost 1983; 50(03): 686-689
DOI: 10.1055/s-0038-1665288
Original Article
Schattauer GmbH Stuttgart

Standardization of β-Thromboglobulin and Platelet Factor 4: A Collaborative Study to Investigate the Sources and Extent of Variation in the Measurement of Platelet Specific Proteins

A D Curtis
The National Institute for Biological Standards and Control, London, U.K.
,
P J Kerry
The National Institute for Biological Standards and Control, London, U.K.
› Author Affiliations
Further Information

Publication History

Received 20 June 1983

Accepted 18 July 1983

Publication Date:
18 July 2018 (online)

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Summary

A collaborative study involving seven laboratories has been carried out to investigate the sources and extent of variation in the measurement by radioimmunoassay of β-thromboglobulin (β-TG) and platelet factor 4 (PF4). Three plasma samples, one purified β-TG sample and two purified PF4 samples were assayed against house standards for the respective antigens by each laboratory.

The main source of variation of both β-TG and PF4 measurements was inter-assay (particularly for PF4). It was found that a significant component of this variation was due to the participants’ use of different preparations as house standards for β-TG and for PF4. This indicates that the first step in the standardization of β-TG and PF4 measurement should be the introduction of a reference for each.