Thromb Haemost 1983; 50(03): 740-744
DOI: 10.1055/s-0038-1665299
Original Article
Schattauer GmbH Stuttgart

An Enzyme Linked Immunosorbent Assay for Determination of Tissue Plasminogen Activator Applied to Patients with Thromboembolic Disease

Nils Bergsdorf
The Department of Physiological Chemistry, University of Umeå, and Department of Clinical Chemistry, Umeå University Hospital, Umeå, Sweden
,
Torbjörn Nilsson
The Department of Physiological Chemistry, University of Umeå, and Department of Clinical Chemistry, Umeå University Hospital, Umeå, Sweden
,
Per Wallén
The Department of Physiological Chemistry, University of Umeå, and Department of Clinical Chemistry, Umeå University Hospital, Umeå, Sweden
› Author Affiliations
Further Information

Publication History

Received 13 July 1983

Accepted 11 August 1983

Publication Date:
18 July 2018 (online)

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Summary

Utilizing the immunoglobulin fraction from a goat antiserum against human uterine tissue plasminogen activator, an enzyme- linked immunoassay for tissue-type plasminogen activator in human plasma has been developed. With the new method, the concentration of t-PA in normal human acidified plasma is found to be 4.0 ± 1.8 (SD) ng/ml. It increases to 12 ng/ml after a tomiquet test, and to 14 ng/ml after strenous physical exercise. In a group of patients with idiopathic thromboembolic disease, the resting t-PA concentration was 5 ng/ml and the post-occlusion value 16 ng/ml. Furthermore, the patients also exhibited a normal post-occlusion rise in the concentration of plasmin-α2-antiplasmin complex. However, in 37% of the post-occlusion patient plasmas, virtually no increase in t-PA could be detected by a specific activity assay. The results indicate that the reason for a defective post-occlusion fibrinolytic activity in a majority of cases may be the presence of increased concentrations of a fast-acting specific t-PA inhibitor.