Summary
Primary cultures of human endothelial cell monolayers were incubated with albumin-bound
fatty acids of the ω-3 and ω-6 families for a maximum of 24 hrs, to investigate the
production of 6-keto-PGF1α, TXB2 and platelet inhibitory activity (PIA). Arachidonic acid was a potent stimulator
of all parameters. The release of 6-keto-PGF1α was significantly reduced by equimolar concentrations of linoleic, dihomogamma linolenic
and eicosapentaenoic acids, but not by linolenic acid. PIA was not similarity affected.
Dihomogamma linolenic add was also a weak stimulator of 6- keto-PGF1α and PIA, but reduced the content of both in the cells after 24 hrs. Eicosapentaenoic
and docosahexaenoic acids both depressed 6-keto-PGF1α production but PIA was maintained after 24 hrs. Indomethacin always blocked 6-keto-PGF1α and PIA production. None of the effects correlated to release of 51CR from prelabelled cells.
Keywords
Endothelial cells - Polyunsaturated fatty acids - Platelet aggregation - 6-keto-PGF
1α
- TXB
2
