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Thromb Haemost 1983; 50(04): 835-837
DOI: 10.1055/s-0038-1665323
DOI: 10.1055/s-0038-1665323
Original Article
Cell-Free Synthesis of Factor VIII Related Protein
Further Information
Publication History
Received 26 May 1983
Accepted 27 September 1983
Publication Date:
18 July 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/198304/lookinside/thumbnails/10-1055-s-0038-1665323-1.jpg)
Summary
Polyadenylated ribonucleic acid (RNA) was isolated from confluent human endothelial cells in culture and translated in a cell-free rabbit reticulocyte lysate protein-synthesizing system. Using specific immunopredpitation, the translation product was found to contain 1.58% VIH related protein (VIIIR), which has a molecular weight of 0.26 × 106, similar to the subunit of VIIIR present in normal plasma. This indicated that VIIIR messenger RNA is responsible for the synthesis of subunits only and intracellular post-translational events are required for the assembly of tetramer and multimers.
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References
- 1 Nilsson IM, Blombäck M, Jorpes E, Blombäck B, Johanson SA. Von Willebrand’s disease and its correction with human fraction I. Acta Med Scand 1957; 52: 179-188
- 2 Cornu P, Larrieu MJ, Caen J, Bernard J. Transfusion studies in von Willebrand’s disease: effect on bleeding time and factor VIII. Br J Haematol 1963; 9: 189-202
- 3 Tschopp TB, Weiss HJ, Baumgartner HR. Decreased adhesion of platelets to subendothelium in von Willebrand’s disease. J Lab Clin Med 1974; 83: 296-300
- 4 Zucker MB. In vitro abnormality of the blood in von Willebrand’s disease correctable by normal plasma. Nature 1963; 197: 601-602
- 5 Howard MA, Firkin BG. Ristocetin - a new tool in the investigation of platelet aggregation. Thrombos Diathes Haemorrh 1971; 26: 362-369
- 6 Rick ME, Hoyer LW. Immunological studies of antihemophilic factor (AHF, factor VIII). V. Immunological properties of AHF subunits produced by salt dissociation. Blood 1973; 42: 737-747
- 7 Jaffe EA, Hoyer LW, Nachman RL. Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J Clin Invest 1973; 52: 2757-2764
- 8 Jaffe EA, Hoyer LW, Nachman RL. Synthesis of von Willebrand factor by cultured human endothelial cells. Proc Natl Acad Sci USA 1974; 71: 1906-1909
- 9 Hoyer LW, Shainoff JR. Factor-Vlll-related protein circulated in normal human plasma as high molecular weight multimers. Blood 1980; 55: 1056-1059
- 10 Ruggeri ZM, Zimmerman TS. Variant von Willebrand’s disease. Characteriza-tion of two subtypes by analysis of multimeric composition of factor VIII/von Willebrand factor in plasma and platelets J Clin Invest 1980; 65: 1318-1325
- 11 Ruggeri ZM, Zimmermann TS. Classification of variant von Willebrand’s disease subtypes by analysis of functional characteristics and multimeric compo-sition of factor VIII/von Willebrand factor. Ann NY Acad Sci 1981; 78: 205-209
- 12 Chan V, Chan TK. Characterization of Factor VIII related protein synthesized by human endothelial cell: a study of structure and function. Tbromb Haemostas 1982; 48: 177-181
- 13 Nakanishi S, Taii S, Hirata Y, Matsukura S, Imura H, Numa S. A large product of cell-free translation of messenger RNA coding for corticotrophin. Proc Natl Acad Sci 1976; 73: 4319-4323
- 14 Favaloro J, Freisman R, Kamen R. Transcription maps of polyoma virus-specific RNA. Analysis by two-dimensional nuclease Sl gel mapping Methods Enzymol 1980; 65: 718-749
- 15 Aviv H, Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci USA 1972; 69: 1408-1412
- 16 Kessler SW. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol 1975; 115: 1617-1624
- 17 Maniatis T, Fritsch EF, Sambrook J. In “Molecular cloning”. Cold Spring Harbor Laboratory; USA: 1982. p 189
- 18 Chirgwin JM, Przybyla AE, McDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 1979; 18: 5294-5299
- 19 Chan TK, Chan V. Antithrombin III, the major modulator of intravascular coagulation, is synthesized by human endothelial cells. Thromb Haemostas 1981; 46: 504-506
- 20 Moncada S, Higgs EA, Vane JR. Human arterial and venous tissues generate prostacyclin (prostaglandin X), a potent inhibitor of platelet aggregation. Lancet 1977; 1: 18-20
- 21 Weksler BB, Marcus AJ, Jaffe EA. Synthesis of prostaglandin I2(prostacyclin) by cultured human and bovine endothelial cells. Proc Natl Acad Sd USA 1977; 74: 3922-3926
- 22 McDonald RI, Shepro D, Rosenthal M, Booyse FM. Properties of cultured endothelial cells. Ser Haematol 1973; 4: 469-478
- 23 Lynch D, Zimmerman T, Kirby F, Livingston D. Studies on the biosynthesis of bovine and human von Willebrand factor (Factor VIIIR). In: “Symposium on Factor VHI/von Willebrand factor”. San Diego; USA: 10/1982. p 12
- 24 Wagner DD, Marder VJ. Biosynthesis of von Willebrand protein by human endothelial cells. J. Biol Chem 1983; 258: 2065-2067