Thromb Haemost 1983; 50(04): 838-843
DOI: 10.1055/s-0038-1665324
Original Article
Schattauer GmbH Stuttgart

Platelet Function, Size and Yield in Whole Blood and in Platelet-Rich Plasma Prepared Using Differing Centrifugation Force and Time in Domestic and Food-Producing Animals

R M Clemmons
*   The Department of Medical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, WA, U.S.A.
,
E L Bliss
**   The Department of Preventive Medicine, College of Veterinary Medicine, University of Florida, Gainesville, WA, U.S.A.
,
M R Dorsey-Lee
*   The Department of Medical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, WA, U.S.A.
,
C L Seachord
***   The Laboratory of Comparative Thrombosis and Hemostasis, College of Veterinary Medicine, Washington State University, Pullman, WA, U.S.A.
,
K M Meyers
***   The Laboratory of Comparative Thrombosis and Hemostasis, College of Veterinary Medicine, Washington State University, Pullman, WA, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 10 March 1983

Accepted 29 September 1983

Publication Date:
18 July 2018 (online)

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Summary

The effects of centrifugation force and time upon platelets function, mean platelet volume and platelet yield were compared with whole blood platelet counts and size in citrated blood samples from the bovine, canine, caprine, equine, feline, ovine and porcine species. The results were similar, for a given species, irregardless of sample volume. Bovine, caprine, feline and ovine platelet yields and mean platelet volumes were maximal when platelet-rich plasma was prepared using longer centrifugation times and lower gravitational forces. Canine, equine and porcine platelet yields and mean platelet volumes were maximal when platelet-rich plasma was prepared using shorter centrifugation times and higher gravitational forces. Platelet aggregation to adenosine diphosphate or arachidonic add was not effected by the method of platelet-rich plasma preparation in bovine, caprine, feline, ovine or pordne platelets. Equine platelet aggregation was maximal when platelet-rich plasma was prepared using longer centrifugation times and lower gravitational forces. Canine platelet aggregation, particularly arachidonic add-induced aggregation, was maximal when platelet-rich plasma was prepared using short centrifugation times and higher gravitational forces. It appeared that the effects of centrifugation parameters upon platelet yield depended upon the relative difference between platelet and red blood cell volumes.