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Thromb Haemost 1997; 78(06): 1520-1526
DOI: 10.1055/s-0038-1665445
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Up-regulation of Tissue Factor Expression by Platelet-derived Growth Factor in Human Vascular Smooth Muscle Cells in Culture

Role of Mitogen-Activated Protein Kinase and Effects of Intracellular Cyclic AMP
J M Xuereb
1   The Laboratoire de Recherche sur I’Hémostase et la Thrombose, Hôpital Purpan, Toulouse, France
,
P Sié
2   Faculté des Sciences Pharmaceutiques et Biologiques, UPS, Toulouse, France
,
B Boneu
1   The Laboratoire de Recherche sur I’Hémostase et la Thrombose, Hôpital Purpan, Toulouse, France
,
J Constans
1   The Laboratoire de Recherche sur I’Hémostase et la Thrombose, Hôpital Purpan, Toulouse, France
› Author Affiliations
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Publication History

Received 02 1997

Accepted 16 July 1997

Publication Date:
12 July 2018 (online)

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Summary

Human vascular smooth muscle cells (SMCs) isolated from normal adult arteries were investigated for the expression of Tissue Factor (TF) procoagulant activity at their membrane surface and TF antigen in lysed cells. In growing conditions, SMCs expressed high levels of TF activity and antigen. When cells were made quiescent by 72 h of subculture in serum-poor medium, these levels fell to about one third of the initial values. Platelet-derived growth factor BB (PDGF) up-regulated in a dose-dependent manner TF expression with a significant increase (1.8 fold) within five hours. PD 98059, a specific inhibitor of mitogen-activated protein kinase (MAPK) pathway involved in cell response to PDGF, also prevented TF up-regulation. Short-term treatment by Pentoxifylline and dibutyryl cAMP also completely prevented induced TF up-regulation, but remained without effect on baseline levels of quiescent, unstimulated cells. At their effective concentrations, pentoxifylline and dibutyryl cAMP also inhibited the activation of MAPK induced by PDGF. The rapid induction of TF upon growth factor stimulation may be important in circumstances where SMCs proliferate, such as vascular events or vessel development.

 

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